FastQCFastQC Report
Thu 24 Dec 2020
HK3T5AFX2_n02_AA01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n02_AA01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG33.6585365853658534No Hit
CTGCTGGAGTAGAATAACTGGATGAGTTTACACTACACTCTAGGTCACGT11.2195121951219512No Hit
TTTCAATGCCGTTCTTCTTTGAAGCCTCCAGTTGTCTCCACTAGAACCTA11.2195121951219512No Hit
GTCCCATGCTATGCAAGGTCTGGAAGAAAAATTTTTGAGTTAGTGGGGTC11.2195121951219512No Hit
GCCGTGGCCCGGATGTCGTGGATCCGTTGTTGGCTCGTACTTTGTAATGC11.2195121951219512No Hit
GTTGTATAATAGAAAATGGGTTGAGGTAGAAAATGGTAATTAACTAGAAT11.2195121951219512No Hit
AGTGTGGACAATCGGACATTACTTCGCGCCGAGACCCTTCCACAAACCGT11.2195121951219512No Hit
GTGTGTATCTGGTTTTGGAACGAGGCGGCCAAAGTTGCATTCAACTTTAA11.2195121951219512No Hit
NNNCTTATGCGAACCCGGGCACTCGGTAGCCAGGCTGCCGCTCCAGGCCT11.2195121951219512No Hit
TTGTTAAACTTATCCGAATGATTGGCAAAAGTGAAGAACATCGGATAAAT11.2195121951219512No Hit
TTAAACTTGACTTAAGTAACTCTCACAGTCTCCCGACGAACAGGGAGAGA11.2195121951219512No Hit
GTGTACAAGAGAAAATGGGTCCGCGGAAAGCAGGGATATTGCCTAGAACA11.2195121951219512No Hit
ACTATAGACAGCATAAAAAATATATTTTTCCAGGTGGACCTACTATCTGC11.2195121951219512No Hit
GGAGACATCTCGCAATAGGAGGTGTGTATATAGGGGGGACAGAAGAGGCG11.2195121951219512No Hit
CATTTACATTGTGTCAGTGAACCCAAAAAAACTTCACCTCATAAAAACTT11.2195121951219512No Hit
AAACTGATGTTCAAAGTTAGAGACAATTTCATAAGTCCGAAGCGGTGTAG11.2195121951219512No Hit
CAGTTCTCATAATCACACACAATCGTACGTCTACGTGTATCAGAGGAGAT11.2195121951219512No Hit
GGTATAGGCCTAGGCAGGCAGTCCTCGCAGACGCTTATAATAGTCATTAA11.2195121951219512No Hit
GTCCAATACCAGAGCCACCCATCTGACCACTGTTTGACCTGAACCAACTG11.2195121951219512No Hit
CTCCTAGTATTAAATTTTCTATAAACAGTAAAAACCACTAAAGTAGCAAA11.2195121951219512No Hit
GCACATGGCTGAGCACGTTTCCTCTCCTTGAAAACACGCATCTCAACTAC11.2195121951219512No Hit
GGCTGGGGGGGGGGGTGGGGGGGGGGGGGGGAGGGGGGGGGGGTGGCTGG11.2195121951219512No Hit
GTTGAATAGTGCCACGCCAAGACAACGGCTTGATGAGATATTTCAATCAC11.2195121951219512No Hit
GAGTTGGCTCGCGTTCACGACCAAACAAGTTGTCACTTGTTGTGGCAGCA11.2195121951219512No Hit
GGCTTAGGCTTAGGCTTAGGCTGAGGCTTAGGCTTAGGCTTAGGCTTAGG11.2195121951219512No Hit
CGCCAAGCCCTCGGAGCCCACCGTCGAAGCGGCCAGGTTCGACGATCTGC11.2195121951219512No Hit
AAAACAGCACCTACAAGAAACAAAAAAATAGCTGCCACAGAAAACTTAAA11.2195121951219512No Hit
GTATTATTCATAAATTCTATTCATGTGTCTCAGTTATAGCGATTTACTCA11.2195121951219512No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGG11.2195121951219512No Hit
TTGTAATCTTCTCATTAGAATTTCAGTTTCAGTTGACCGCGAATGTGAAG11.2195121951219512No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.2195121951219512No Hit
AGTTATGATCATGTGATGCTACTGGCCGAGCTCGACCGACAAGCAGGTAG11.2195121951219512No Hit
GTCCTGAAGTCGGTCTGCAAAGCCTGGTGCGAAGGCTTCTGGGCTGTCTC11.2195121951219512No Hit
CAGTCACACAGTGTGTCCTGAAGTGATTGTCGCAGAAATGAGGCACACGA11.2195121951219512No Hit
GTCATTAAGTGGCTCTTCTGCTAGACGGAAGTTACAAATCATTGCTTCGG11.2195121951219512No Hit
ACACACACATCCGCGGGACGAGCTCGTTCGGAGATCGACGACAATTCCCC11.2195121951219512No Hit
ATTTTATACAGAACACACTGTATATTTGCGCAGATATGTCTCATACGTTG11.2195121951219512No Hit
GAATACGGGAGAGCGGCCGGGCGGCGGGGGGGGGTCGGAGGGGCGGGGGG11.2195121951219512No Hit
CTATTATTCTATTCTCGTCTGGTTGGAAGAGCATGGAGACAGTTTCCAGT11.2195121951219512No Hit
ACAATATCAACTAACAAAACAAATTCACACCGCCTCCCGCGCCACACAAA11.2195121951219512No Hit
CAGAATATGGTGCAATATCATTCAGACATTCAATCTCTCTACCTAGATTC11.2195121951219512No Hit
GTGTAAAATACGTGGTAATCGAGGGGGGAGACGAGGTGAGCAAACGCGCT11.2195121951219512No Hit
ACCTTGACGAGAGCGACTGGTGGATCGTTAGATGAGAGTTTTGGGGCAGC11.2195121951219512No Hit
GGGGGGGGTGGTGTAGGAAGGAGTGATGGCGGGGGGGGGGGGGGGGGGGG11.2195121951219512No Hit
ATGTGATGTCCTGCGTGGAGGGGGGGGGAGGGTGCGGGCGGCTGAGTTCG11.2195121951219512No Hit
GCCTCTAGTCGATAGAATAATGTAGGTAAGGGAAGTCGGCAAACTAGATC11.2195121951219512No Hit
CTGTTATATGTGGAACAAAATTAAATGTCGGCAAACTTTCCTACAAATTT11.2195121951219512No Hit
CCTTTTAGTCAAATTGACACACACCTAATTTACTCTATCTAGATGATCTC11.2195121951219512No Hit
GAGTGGACACTGGTAGTCGGTAATGTGTCAATACACGCGATGTGCACTAA11.2195121951219512No Hit
GATCGGGAATCTGTCGAGGCGGCTACGGCGTTTAATGTGAATTTTTCGTT11.2195121951219512No Hit
TTCTTACATGTTGAATGTTGGTTTCTGAAAAATGAAAATGAATTCGAAGA11.2195121951219512No Hit
GTGGGTACAGGTCGGTAATGTCGAGGAATGACAAAGGTTTACCACAAAGT11.2195121951219512No Hit
GTTTTGGAGCGGTATTGGAACGGTTTTGGAGCGGTTATGGAGCGGTATTG11.2195121951219512No Hit
AAATAGAACGCAACAACAATAAAACTAGTATCTTTAAGCGACACACAGAT11.2195121951219512No Hit
CCTCAATATTCCGAAGCAGCTAAAATAATCAGAGAGACGTGTGACATTGC11.2195121951219512No Hit
ACCTAGTACAGACCTCTCCAATTACCCTATGAAGAACAAAAAAAGCTCGA11.2195121951219512No Hit
CGCTCTGTCTGTGCAGAAAAAGATGACCTCCCTCTCTAGTCCTAGACCCA11.2195121951219512No Hit
CCCCCCAACCCGAAAAACCTACCTCGCGTGACGAATACCCCCACTAGCAC11.2195121951219512No Hit
CCAATTAGAAGTCGTCTTTGAGAAGCTAGGTAGACGACAAACACCATGAG11.2195121951219512No Hit
CACCTGTACTGCCGACGAGCATTGAGCGTTGCCGTCGAGCCCATCATATA11.2195121951219512No Hit
GCACAAGGGCACGCGGACGAAAGAGGCTAGGGGGGGCGAGGCGCAAGTCG11.2195121951219512No Hit
ATAACTTTTTATATTCCAACATATACCCTATTATCTACTATATGTCGTCA11.2195121951219512No Hit
GTGGAGATCTAGTATCGGGAAATCACCACAACAGACAGCAAAACGTCTCT11.2195121951219512No Hit
ATACTGAACCGCGCGCGCCGCCGTCGGATGACAGGTGTCTCTTATGCGCA11.2195121951219512No Hit
CATAAATCCTAACCACTAGACAACAACGGAGGTGATAAGCACTCATTATA11.2195121951219512No Hit
AAAACAAACAAATCCCACAAACAATTTTTGTTTTTATCAGGATATGCGGT11.2195121951219512No Hit
ATTTTTGGGTTGTCATTGTAAGTGCAACAAAAAAATAGTTTAATGTTTTG11.2195121951219512No Hit
GCGTGTCGCGTCGCGGCTCGAGTTTAGTTGTAATATTAAATGTATTTGTC11.2195121951219512No Hit
GCGGTGGCCTGCGTGTCTAGCGGTCGAACATGTTTTCTCTGCGGCCTCTT11.2195121951219512No Hit
TGTCTCATTAGTGTCCAGCAAAAAATTGAGCCAGGTGGGGGTACTATAGC11.2195121951219512No Hit
GTTAAATGTGAAGAAGGCGGAGGCGGATTTGCAATTGGCCAATGCAGAAA11.2195121951219512No Hit
GGTCGTGCGAGACCGATAAGCCTTAAATGTAGAATGATAGGAAGATTAAA11.2195121951219512No Hit
CATTTGACCTGAGCAATGTTGTGTGGGCGTTTGGGCGGCTGAGCTGTGGG11.2195121951219512No Hit
CTCTCGTCCTATGCCGATGACAACTCGAAGGGCGGGGGGTCGGGGCGGGG11.2195121951219512No Hit
GGCGAGGGGGGCTCGGGGCAGAGGAAGGCGCCGCGGCGGGGGGTTGTTGT11.2195121951219512No Hit
GCATAACTCTGCTCAAAAAAATTCTCCAATCTCGGCCGGACGGTGGTGCT11.2195121951219512No Hit
CCAGTTATCTGTCTTTATCGACGATTAGAATGTCTTAGAATCCTCTATCT11.2195121951219512No Hit
ACATAGTGATTTACAAAAACAAGAAGAAACTTAAAATAACATTTCCGAAT11.2195121951219512No Hit
GTTCAGTCCCGGATAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAA11.2195121951219512No Hit
GGCTCCGCGGACTAATAGGGAATTACGCAATGCCCTCGAGGTTCCAGTAT11.2195121951219512No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAT50.070.061
TGCATAT50.070.063
CACACTG50.070.058
GTGTCCA50.070.011
TAGTGTC50.070.09
CTATAGC50.070.044
CACACAC50.070.056
CAGCAAA50.070.016
AGCCAGG50.070.029
TTACCTA50.070.069
GCAAGCG50.070.049
ATTAGTG50.070.07
AAATTGA50.070.023
CCAGCAA50.070.015
TGTCTCA50.070.01
TGTCCAG50.070.012
GGGTACT50.070.039
GTACTAT50.070.041
ACACTGC50.070.059
GAGCCAG50.070.028