FastQCFastQC Report
Tue 5 Jan 2021
HK3T5AFX2_n02_AA01_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n02_AA01_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7378330
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC106350.14413830772003963No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG155400.021.38991270
CCGACGA209200.020.23765270
GACGAGC101100.018.44643470
GCCGACG280950.017.94615470
ACGAGCG61100.016.37801470
CTATACT23700.015.5318534
TACCGGC23000.015.36490852
TGCCGAC338950.015.36033769
ATACCGG23300.014.86643651
CGAGCGA47150.014.47069370
TTATACT27750.014.2757114
TAATACT30100.013.9764564
TATACCG13300.013.9622635
GGCAGTA26950.013.89255256
CTGCCGA386050.013.50461168
CTAGTAC17500.013.030783
GAGTCCT30350.012.91180438
TATACTG31800.012.8911115
GCTGCCG407700.012.75332367
TCATACT30850.012.7275594
Produced by FastQC (version 0.11.5)