FastQCFastQC Report
Tue 5 Jan 2021
HK3T5AFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16814904
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3457232.056050989051142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGTA4500.020.22054322
CTATACT6650.019.4737454
TACCGTG3450.019.2731887
GTACCGG5300.017.82860226
AGTACCG3850.017.2727815
GTACCGT5300.017.1698676
TACACAG9250.017.027085
TAATACT11150.016.3229224
TACCGGA5700.015.96396527
TAGACAG8050.015.6522235
GTCCTAA2704.3804903E-815.5638891
TGCACTG9000.015.5556055
AGACAGT8300.015.1807716
TATACTG6150.014.7967955
TAGACTG5000.014.7000475
GTGTAGA10250.014.6907931
TAGGACC2151.9785557E-514.6512094
ACCGTGT4550.014.6137358
ACTGTCC7000.014.49836358
TACACCG5100.014.411815
Produced by FastQC (version 0.11.5)