FastQCFastQC Report
Tue 5 Jan 2021
HK3T5AFX2_n01_DJ78_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n01_DJ78_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5756401
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG142380.24734204583732092No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT17450.018.6543674
TCTATAC16800.018.5427323
CTACACT27500.017.9464864
TATACTG22250.017.1470535
TACACTG31550.016.752155
TTATACT21500.016.6056064
CGAGACT56650.016.18691870
GTCCTAT9850.015.9975771
ACGAGAC85400.015.98341570
GTAGGAC9650.015.9594663
TACACTC25600.015.8602875
CCACGAG131300.015.78046570
CACGAGA111900.015.73260970
ACTGTGC23300.015.4719168
TTACACT29050.015.4225724
TAGCACT13200.015.3796724
TATACTT21350.015.2467785
TACACCG16800.015.2092085
CCGTGCG7600.015.19718559
TAGACAG22000.014.6372045
Produced by FastQC (version 0.11.5)