FastQCFastQC Report
Tue 5 Jan 2021
HK3T5AFX2_n01_DJ77_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n01_DJ77_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8558846
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG208350.24343235057623422No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC177420.20729430112424035No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGA247650.018.58424870
ACGAGAC177100.017.64779370
CCACGAG308400.016.69382570
CGAGACA129050.016.59781670
CTATACT22450.014.8131014
CCCACGA356650.014.62182569
TATACCG14100.013.9029715
CCCCTGG11600.013.2854751
TATACTC26200.013.22734455
TGGACCG16500.013.1536575
TAATACT26000.013.0598174
GCCCACG400950.012.98010768
TATACTG28950.012.8172855
GTATAGA20000.012.7842511
TCTCCGA1009350.012.73610670
CTAGTAC22050.012.5417243
GTTCTAA22150.012.3339811
TTATACT27300.012.3096964
CTCCGAG815800.012.27844870
GTATTAA19750.012.2367031
Produced by FastQC (version 0.11.5)