FastQCFastQC Report
Tue 5 Jan 2021
HK3T5AFX2_n01_AA18_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n01_AA18_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3497819
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG85130.24338023208176296No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG84250.24086437863137No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC216100.024.99037770
CACGAGA323400.024.014870
CGAGACG137250.020.55343270
CCGTCTA4650.020.322289
CTATACT6850.019.9289914
TACTAAG2555.4569682E-1119.2176022
CCACGAG434400.018.41018170
CTTATAA13400.018.02212370
TTACACT9350.017.9697064
GGACCGT7700.017.2744466
ACTGCGT11650.017.1242128
TTAGGAC4100.017.0748713
AGGACCG5800.016.8982355
TACTAAC5600.016.876682
TACACCG12350.016.7223155
TATACTG7600.016.58065
TAGAATA5700.016.5805994
GAGACGA100650.016.4826270
GACTAGC3401.0913936E-1116.47034611
TATACTT5550.016.3980315
Produced by FastQC (version 0.11.5)