FastQCFastQC Report
Thu 24 Dec 2020
HK3T5AFX2_n01_AA17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n01_AA17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGTACTTATAGTAAGGTAAGTTTTACATCGATGTTGTATTCAGATA11.7543859649122806No Hit
GCCTAAGCCAAAGCCTAAGACTAAAACTAAGCCTAAGCCAAAGCCTAAGC11.7543859649122806No Hit
GACACTGAAAAAAAAACAAAATTATAGAAACTCGCAGTACAACAAAAAAA11.7543859649122806No Hit
GGGGGGTAGTGCGCGCGTTGTCTGCCGTCTCTCAAATTGCACACTGCCGG11.7543859649122806No Hit
GTCTTTAACAGCGTCTTTGTGTTGATTAACAAGCTGAATAACTGAATCCA11.7543859649122806No Hit
ACTTGAGGGAGTACGGTAGGTAAGGGCAGATGATACCAATCCATTGTTGG11.7543859649122806No Hit
ATCAAATTTGGGTCCCGCCACGAAAACTAAAGTAATCCAAGTTTTCAAAA11.7543859649122806No Hit
AATTAAAACTGCGTTTATAGTGCATTTGCTTCTAGATGACAAGAGGTCCC11.7543859649122806No Hit
GGGTGGCCTCGCCTGACAAACTATCATATTACCACACAAAACTGACCGTG11.7543859649122806No Hit
CTGCCTACCTGCAGCCTACTTCTGCATATTTAATGTGAAATCAGGTATGT11.7543859649122806No Hit
ACAGAACACTCCTAAAAAAATAAAACAATAAAAGTACAAATAACTAAAAC11.7543859649122806No Hit
CTATTTCGTATCGTAAATAGCTATATCGTATAGTTTATAGCTATATCGTA11.7543859649122806No Hit
GGGGTAGGGTGGGCGGCTCTAGGCGGTGCCGCCCTTGTGTCGGAGGAATG11.7543859649122806No Hit
GACTAAAACACCACACAAAATTAAAATCGCTAATGAAACTTTGAGCGGCC11.7543859649122806No Hit
GTAACATGCACTAGACAGGTAAGTATGTACAGGTCAGTATGTAGGCATGT11.7543859649122806No Hit
CACACACACACACAGAAAAGAAGAGAAAGAGCGGTTTAGAGTAATGAGAG11.7543859649122806No Hit
AACCGTAACCCGGCTGTCCGCACCACTACCGCAGCACCAAAATACTACAC11.7543859649122806No Hit
GGCATAGGTTTATGCTAAGGCTTAGAACTTGGCTTACGCTTAGGCTAAGG11.7543859649122806No Hit
CATCAATGGTCTAGGAACCTTAACTGGCGAAGGAGCCTTTACGGGCTTAG11.7543859649122806No Hit
CATCAACAACTGGGAAGACAATCCAATGGAATATCAATTTCACCAAACGC11.7543859649122806No Hit
GCCCCGAACTGCTCATCTCCCGGCCCTACTTGATCGACGCCCTGCTCGCC11.7543859649122806No Hit
CACATACATTGGATCCTGCAATTCCGACTGTTGCGTAAGCTGTCTCTTAT11.7543859649122806No Hit
GAGGGGAGGTTACACTAAGAAATAAAATGGTGGGGAAGATATAATTGCGC11.7543859649122806No Hit
TGATATTTGAAGTAGTCACATCTCCAACTGTCCATTCTGGATGCTCCGCT11.7543859649122806No Hit
GGGGAGGGGAGGGCGACAGGCCCTAAGCGCCCGGCGAAGGCCGTACCCTA11.7543859649122806No Hit
AACACGAACCCCGCAGCAAACCGAGCTGCAAACACTGCAGGGCTGTCTCA11.7543859649122806No Hit
CAACCGGCTCCGGCAACCAGTCCAACTACCCATCCTACTCCTGCTTACAC11.7543859649122806No Hit
CAAAATTTTACATAAACAACAAAAAAATACCCATCCAGCAAAAACAAAAA11.7543859649122806No Hit
GGGTTAGTCCCGCGACCTCCCGCGCAGCCAACGCCGCAGTAAACAGTGGG11.7543859649122806No Hit
CTGTCCCACTCACTCACTGAGCACGGGAGGACGAGGTCTCCAACGACCGA11.7543859649122806No Hit
ATTCAAAACACTGACAAATGCGCTGGCAAATTGTCCACAATGCAAGAAAT11.7543859649122806No Hit
AACCTACGTCGAAGCTTACGAACAACTGGAAATTGCACTTTTGGAGCCAC11.7543859649122806No Hit
CAGCTAAAGTACACCACAAAAAAAGTAGCATCCAAGAAACGATATTACAG11.7543859649122806No Hit
GTTCAGGAAACCCTACTCGATACTCAACAATAGCTACTTACACTCCCACT11.7543859649122806No Hit
GGTCCGCCGCATGCCCACCGCCCCCGCCCAGGCCGCCGCCCCCACCCGCC11.7543859649122806No Hit
ACAAACACCCGCCAACGAACACATCAAGAACCAAACAAAACAAACAGAAA11.7543859649122806No Hit
GAGTGTGTCTGTGTGATTTTCCGTGTTTTGCGTTGAATTTTTCAGAGACG11.7543859649122806No Hit
GCATAACTCTGCTCAAAAAAATTCTCCAATCTCGGCCGGACTGTGGTGCC11.7543859649122806No Hit
CCGTCGACCAGGTCACCGTACAGGACGCGGACCCGGCGGAGCCAGTCCTG11.7543859649122806No Hit
AGTTACAGCCGTTTGAAGAACCTCTTCCCCTTTCAACGTTTGCTGTTTTT11.7543859649122806No Hit
GCCCCTACCTGATAAAACTGATATTGCGCCGCATTTGTCAGATTAGTCTG11.7543859649122806No Hit
TATCCAATCAGACTCTATTCCTAAAAATCACCAACAAGCAAGAACTTTTC11.7543859649122806No Hit
GGGATCCTACAGTAAGGCTACAGTAACCCTACAGTACCCCTAGCGTACCT11.7543859649122806No Hit
AGTGTCAACAAAAAAATAAGTCAATGATAGGGCTGCCGAAAGGTCGACCT11.7543859649122806No Hit
TCACAAGAGTCAAGATGCCGGAAAGCAAAAGGGATCCAAGGATGGTTAAG11.7543859649122806No Hit
GATCTACAGTGCTCTACATTTCTTGAAATGTTGAATAGTTAATATTTTCA11.7543859649122806No Hit
GTGCATTGCTTAACATAGCATTACAAGAGAAAATACCTACAACACCAACT11.7543859649122806No Hit
GCCTAGGCCTAAGCCTGATTCTAAGCCACGTCTAAGCCTGAGCCTAAATC11.7543859649122806No Hit
GTCTACACCACTTTCTACAAACAAAACAACACAACCCACAAAAGACGGAA11.7543859649122806No Hit
ATATCAAAGCTCAACACAAAACCAAAGAATACAGCCAACTGAACTACAGC11.7543859649122806No Hit
AAATATAAATTTAATTAAGTAAAAAAAAAAAATAAAAATACAAAAACAAT11.7543859649122806No Hit
CCACCAAAGTCACAAAATGAAAGAAAATTTAGTGCAAGCCGCAGAGGTCT11.7543859649122806No Hit
GCAAACACTTTTCACTCATCATTAAGCCCTGACACAAAAGAAGTTTAGGA11.7543859649122806No Hit
CCGGCAAACCGGCAAATTTTCAATTCCGGCGATTTGCCGAGTTGCCGGAA11.7543859649122806No Hit
GTCTGGCGTCTATCGCTCTCTCTCCGCATGACAGCCTGCGTTTATTGTGC11.7543859649122806No Hit
ACGGGAGACAGAAAAACAGTGGAAGATCAAACAGTGAGAGAAATATCATG11.7543859649122806No Hit
ATGTCCACACTTTGCGGTCGATCAATCGATGCATCCATCTCACTTCTGTC11.7543859649122806No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCTT50.070.060
CTTCAGC50.070.064
AACAATA50.070.026
AAACCCT50.070.08
CCTACTC50.070.012
ACTCGAT50.070.015
TCCCACT50.070.044
CAGCATC50.070.067
CGATACT50.070.018
CGATAAC50.070.055
GATACTC50.070.019
TCGATAC50.070.017
GAAACCC50.070.07
CTCCCAC50.070.043
AACTGCT50.070.059
TCAGGAA50.070.03
AGCTACT50.070.032
TAGCTAC50.070.031
TTACACT50.070.038
GATAACT50.070.056
Produced by FastQC (version 0.11.5)