FastQCFastQC Report
Tue 5 Jan 2021
HK3T5AFX2_n01_AA17_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n01_AA17_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8982741
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG385150.42876667600680013No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC229190.25514483830714924No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC330300.022.9302270
CGAGACT204050.021.42324870
CACGAGA466250.020.61299970
CTATACT22500.018.0470034
TTATACT25500.016.8847474
CCACGAG599550.016.74222870
TATACTG27650.015.8250285
TACACTG49150.015.5261075
CTTATAA31050.015.44253170
CTGGGTA45150.015.42605570
TATACTC24400.015.3505375
GTATTAA17700.015.2342541
TCCTATA20800.015.1464632
TACACCG22600.015.0242535
AGACTGC112100.014.98632470
TTACACT32250.014.9788684
TATACAG24300.014.8374945
TATACTA14650.014.5754445
TAGACAG30300.014.4409915
TAATACT29150.014.2901914
Produced by FastQC (version 0.11.5)