FastQCFastQC Report
Tue 5 Jan 2021
HK3T5AFX2_n01_AA16_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n01_AA16_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6811945
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG461820.6779561490881092No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC345480.5071679234051362No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT19050.026.092724
TATACTC19350.022.7937375
ACGAGAC373950.022.62153870
TATGCCG21250.022.56423849
CTCGTAT20750.022.26459945
TTACACT21900.021.2585434
CACGAGA534500.021.03885370
ATGCCGT21250.020.7525150
CGAGACC265350.019.7320270
TCGTATG23800.019.55841846
CGTATGC25500.019.4897947
TACACCG18400.019.404765
CTAGCGC13550.018.85569635
TATACCG7800.018.399895
GTATAGA16250.018.3198181
GACCTAT15150.018.2629131
TATACTG18700.017.7831215
TACTCCG19100.017.77457
GTCCTAT9100.017.7040271
TTATACT14000.017.5023354
Produced by FastQC (version 0.11.5)