FastQCFastQC Report
Tue 5 Jan 2021
HK3T5AFX2_n01_AA14_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n01_AA14_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4724355
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG217600.4605919749891784No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCCTGTCTCTTAT48290.10221501136133927No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC47520.10058515924396029No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGATA57950.025.30584770
ACGAGAC584350.024.61066870
CTATACT9700.024.5387614
CGAGACG375050.022.96586270
CACGAGA855000.022.4446370
TATACCG6400.021.3304525
TATACTC11550.020.3052465
TAATACT7250.019.7952634
TTAAACT7300.019.659684
TCGTGAT80750.019.41760869
TCCTATA6950.018.6351282
GACCTAT6800.018.540461
TCATACT11900.018.5314354
GGTTAAC4000.018.3859581
GGGGTTA5650.017.9665626
ACAGCGT13350.017.8273968
TATTCCG7900.017.7234545
CCTATAC11050.017.4227163
GTCGTGA90650.017.2197768
CCACGAG1125400.017.18869469
Produced by FastQC (version 0.11.5)