FastQCFastQC Report
Thu 24 Dec 2020
HK3T5AFX2_n01_AA13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n01_AA13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGATTCCCAGGCCGTCTCCGATCCAAGTACTAACTGGACTCAACG15.0No Hit
AGTCAGTGGGAATCGACCCGCGCAAACCCCTTTGCAATTAAACAAACATA15.0No Hit
GAGAAATAGTGAGACGGAAAAAGACCAGAGTGAGATGGCTGTGAGCGAGA15.0No Hit
GTAACATTCTTCCTAACTATTTCAAACTTCTTCTCCCAGTTTTTCGATTC15.0No Hit
AAAAAACACACTTTGGCAACCCACCTTAAATAACTATCACAGGCAAACAA15.0No Hit
CTACAGAACGATTCACAGAGTCACAGAGACGTTGAGAAAGTGTACGAACT15.0No Hit
GTTTCGGACTATATTTCAATTTTTTTCGTTCAAAAACATCAGGAAGAGAG15.0No Hit
GACTTGGGCACCTTGTAACCCAAAAGCTCCCCGGTTTCTGTCGTCAGAGG15.0No Hit
CATCAAAACCTTATACAAAAACAAAAAACGAAAAAACATCAACATCCTTT15.0No Hit
CACAGACAGACCTTGAACACTCGAAACACTGGCTCCTTAAATCCCACGGA15.0No Hit
CAATATAGCAACACAAATTTAATGCTCGTATAACTATACACACACATTAA15.0No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCAAAGCCAAAGCATACGC15.0No Hit
ATGTTAGACAGTGCAAAAATCACAAAGCGTTGAAGGGGTACTGTATTTTC15.0No Hit
TTGTACCTTTTACAACTTTTCTAGCACTTCGGACGAGCAAAAAAAGAGAA15.0No Hit
TGACGAGACCCATCGTGGTGAGACCCATCGTGGTGAGACCCATCGTGGTG15.0No Hit
CAATAGCATAGTTCATAGAAAACGTAGACACTCCACCAAATTTGAGTTAA15.0No Hit
CAGTGAAGTCGAGATAAACAAGAAAATGCAAAACTTTCTCCCACATGGAT15.0No Hit
GAGATCAGCAGCACGGTGAACGATCTTCGAGAGAAACTGAACGATTAGAA15.0No Hit
CCTTGTATGTTTTAGGCATCCGTGCGTACAGTCAACCGGTGAGACGAAGA15.0No Hit
AGATCCAGCCAAGGAGGCCCGTACCTTCCACGCTCAGGTTATCATCATGA15.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers