FastQCFastQC Report
Tue 5 Jan 2021
HK3T5AFX2_n01_AA13_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n01_AA13_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6218795
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG351520.5652542011756296No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC275240.4425937822359476No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCCTGTCTCTTAT66430.10682133757424067No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC602550.025.23222770
CGAGACC395150.023.02874270
CACGAGA876050.022.26880670
TATACCG8950.021.5113835
CTATACT14450.021.3178564
TTATACT16700.019.9129624
TATACTG19250.019.820955
TACACCG18500.019.4892085
AGGCTGA80950.019.32633870
TTACACT25650.019.2424534
TACACTG35450.019.1564125
GAGACCG239050.018.6965370
TATACTT13600.018.2746125
CGAGGCT92650.018.20792668
TAGGACA13150.017.835194
CCACGAG1125600.017.51828669
TAATACT15550.017.333614
ATACCGT12450.017.150996
CTACACT34750.017.1246934
TATTAGG11500.017.0459962
Produced by FastQC (version 0.11.5)