FastQCFastQC Report
Thu 24 Dec 2020
HK3T5AFX2_n01_AA07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n01_AA07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGAAAAGTCATGACGGAGCATGATGTAGCTGTAATCATGTTGATGT12.272727272727273No Hit
TCACTGAACTCAAATAAATTAGAATCTCTTCTCATACAATATATGAGCCC12.272727272727273No Hit
GGTGTAACTAGTCGAGCCTCAATGCCTTTCATACCAGCGCAGTCGCTGGC12.272727272727273No Hit
GAAAACAATCCAACCAATACAAGAAAACACATCAAGAAATAATGAACGTA12.272727272727273No Hit
CTACCTCAGGTAGACAACAAGATACACACGAATGTTAAGCACACGAGAGG12.272727272727273No Hit
GGATGGCACTTGTCGCTGCTATGAAGTCGGAAAGTTGGGAGATATCTCGG12.272727272727273No Hit
AAAAAAAACAATTACTAGACAAGACAAACACGAAAAACTTTTATTATAAA12.272727272727273No Hit
GGCTAAGCCTAAGCCTCACCTAAGCATACGACTAATCCTAACATAAGCCT12.272727272727273No Hit
GAGAAATATTGAATGTTCCGCGCCATAAGAGTCTGGTATGTCTGACATTT12.272727272727273No Hit
GCGGACGGTAGGACTACCAAGGGCCCCTATGTGGTTACGGTCGGGGCTGG12.272727272727273No Hit
GCATCAAAAAAAGGTTTCCTAACACTCGTATCGTTGGTCTTTGGGCCTAC12.272727272727273No Hit
AGGAAAATTTAATTAATAAATACATAATAGAAATAACCCAGATTAGGAAC12.272727272727273No Hit
CGGGCCAGGGCGACCCGTTGGGCCTGGCCGCCGGAGAGCTGGGCGGGCTT12.272727272727273No Hit
GTGCGGCCAGTGTCTAGCGGTGTCCATACGCATAGTAGATGGCTTTCATC12.272727272727273No Hit
ACCTAGACTACCTAGACTACCTAGACTACCTAGACTACCTAGACTACCAA12.272727272727273No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG12.272727272727273No Hit
GTCTACCCAGTTAGGGTGACAGCCACCGGGCGACACGTCCCCCGGCCATT12.272727272727273No Hit
CTTTCATAGAAGCGGAGATAGGAGCATCCAAAGTGTGAGGGCGCAGTATA12.272727272727273No Hit
AATCAAATCCGAAACGAAGAAAAAAAACCTACAACTTGTAGACTGACATC12.272727272727273No Hit
GATTAGGGGCAGAATTCGGAACAGGGCCCATGCGCAGATGTATAATATCA12.272727272727273No Hit
TTACAGAAAAGCCGATATATAGACTTGAACACGTTTATAAAATAATTAAA12.272727272727273No Hit
ATCTAAAACATAATCATATAAAAACGCTAACAAAAATAAAAAAAAAAACT12.272727272727273No Hit
GGGTTTGCGACCCTAACGAGACCCTATCCGCACCCGAACCGGTTACAATT12.272727272727273No Hit
ACCTATAACGTCTTACCAGAAATTGTACCAATTATCACCAACCCCATTAT12.272727272727273No Hit
ATAACCAATAAAAAAAATTAATTTTAATTTATTAAAATTATTTCTTAATT12.272727272727273No Hit
CAGTACTTGGTTAAGTGCTGTATACCGAAAAAGCTACACAGTCAAAAATC12.272727272727273No Hit
CACGTGTCACCATGAGCAACATGAGCAAATTCTTATCAGGTGCAACAAAT12.272727272727273No Hit
ATTCGATACTAAGTTAATTGCGAAAGCCTTGTATAATCGTTGTCGAAAAT12.272727272727273No Hit
GAAGGTGACTCGTAATCAGAATAATCATATGATGAATAAGATTTTTTTTA12.272727272727273No Hit
CATATAAATTAATTATACAAAAAAACTAAAGAACTTATAAAATATCTATA12.272727272727273No Hit
TGTCCGGATAGTGTATAATATAACTCATAATAATAATGTTGTATGTTGAT12.272727272727273No Hit
ATTTGGGGCTCACGCTAGTGGAGTTTCGTTTTCACACTCCCAATTTCCCT12.272727272727273No Hit
ATAATTGGTGAAGCCACAGAGAGTAAAATATAGTGGGAAGCCACAGAGAG12.272727272727273No Hit
AATTCGTGAAGGTAACCATTGGAGTATTTAAAGAAGCTTCGAAGGGAGAT12.272727272727273No Hit
GTTTGGGGTCCAATAGAAATGAGACGCGTACAACAGTATATTCCAATGCA12.272727272727273No Hit
GACTAACGGTGGTACTCTGGCTATGAAGGATTCATTGACTGTCGCGAGCT12.272727272727273No Hit
TAAAAGGTAAGGTGTTTGGATAAAACCTTTCTACAAAGATAATATCTAAT12.272727272727273No Hit
CAAATCTTAGCTAACGGTAAAGTAACTCTGTTAAACATATTGTATCAACC12.272727272727273No Hit
GCAGTAATCCTACAGTAATCCTACAGTAATCATACAGTACCTCTACAGTA12.272727272727273No Hit
GTCCGGGATAGGGTATTGTAATTATTGCCCTTAAACGAGGAATGCCTAGT12.272727272727273No Hit
GGTCCACAATCCTGAGAGTATACCTCCTTCCATGGCCAACTTCAGTCTGT12.272727272727273No Hit
CAAAACACAAACATGAACTAATACAATTCAACAAGTATCCCTTATATTCA12.272727272727273No Hit
CTCAATTAGTCGGACAATTTTTCCGTAGAAGTCAATATCCTGATGGCCGC12.272727272727273No Hit
ATAGAAACGTTTAGCGAGAAACAGTTTACTAAGAAACCTATTGCCAAATT12.272727272727273No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)