FastQCFastQC Report
Tue 5 Jan 2021
HK3T5AFX2_n01_AA07_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n01_AA07_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13090888
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG444900.3398547142103729No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC288890.22068021665146015No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT40000.019.2523354
TACACTG73750.018.9853535
TTATACT52100.018.6107034
TAAGGGT28750.018.141334
TATACTG61250.017.8878845
CTACACT63300.017.4744764
TAGACAG47350.017.1509745
TCTACAC66800.017.1353133
TTACACT61850.017.0352124
GTATAAG34300.016.7458721
GTATAGA43450.016.1212331
TAGACTG33450.016.1155595
TACACCG32900.015.9593845
TATACCG24100.015.25081355
CTTACAC46600.015.24863153
CACGAGA201150.015.22465270
CCACGAG225100.015.2218270
TAATACT52950.015.0065424
TATACAG43750.014.9618155
CTAAGGG18500.014.947763
Produced by FastQC (version 0.11.5)