FastQCFastQC Report
Tue 5 Jan 2021
HK3T5AFX2_n01_AA05_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n01_AA05_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12333155
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG449410.36439175539430096No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC372020.30164219942099163No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT37250.020.0159974
TATACTG48100.019.9401975
CTACACT62250.017.6568894
TACACTG64100.017.0926845
TTATACT51000.017.0904354
TAGACTG31950.016.762725
TATACCG23600.015.5740385
TCTATAC35700.015.4921893
TAACACT48150.015.412164
TTACACT57250.015.34695154
GTATAGA38800.015.2557141
TAGCACT31000.015.130984
GTATTAG30750.014.9211991
ACTGTCC39350.014.8535598
TACACAG46200.014.7746295
TAATACT46100.014.7307464
TCTACAC65650.014.7162883
TAGACAG38600.014.69100955
ACTATAC42450.014.67797853
TATACTC48150.014.6124735
Produced by FastQC (version 0.11.5)