FastQCFastQC Report
Tue 5 Jan 2021
HK3T5AFX2_n01_AA04_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n01_AA04_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12234197
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG495850.405298361633379No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC411180.33609071359566955No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT35200.017.50224
TTATACT38050.017.203214
TACACTG67500.016.9576855
TATACTG44400.016.6349725
ACGAGAC166300.016.14216470
CTACACT59300.016.0559774
CACGAGA219100.015.8782570
TTACACT53100.015.7552754
TACACCG30050.015.6092815
CCACGAG251900.015.60307770
TAGACAG41400.015.3038545
TAATACT41750.014.9240284
TATACAG35800.014.7644235
CCTATAC38600.014.6909633
CCTTATA17650.014.6762442
TAGACTG27950.014.4025235
GTACTAA24700.014.3224361
TAAGGGT26600.014.2123114
GTATAGA35550.014.187811
TCTATAC36350.014.0595363
Produced by FastQC (version 0.11.5)