FastQCFastQC Report
Tue 5 Jan 2021
HK3T5AFX2_n01_AA03_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n01_AA03_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6297744
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG68980.10953128612404696No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT14450.019.865914
TATACTG18700.016.4741695
TTATACT16600.016.4493584
ACTGTCC19750.014.7084378
GTATAAG13250.014.0104981
TACACTG28500.013.8802095
TTACACT20450.013.6949084
GTATAAT16500.013.3736571
TCCTATA16550.013.3262632
TATACTC15050.013.0260875
GTATTAA13850.012.8977521
TCTCCGA225700.012.73113570
ATACCGG20450.012.66467351
ATCTCCG261650.012.61381570
ATACTTA15000.012.6027396
CTCCTAT17000.012.568241
GTATTAG12700.012.4108741
TATACTT22050.012.2248795
GTGTTAG14050.012.2155581
TAATACT19500.012.2077814
Produced by FastQC (version 0.11.5)