FastQCFastQC Report
Tue 5 Jan 2021
HK3T5AFX2_n01_AA02_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n01_AA02_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3735801
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG80150.21454568913065766No Hit
AGTCTAGGTAGTCTAGGTAGTCTAGGTAGTCTAGGTAGTCTAGGTAGTCT69600.18630542686829413No Hit
ACCTAGACTACCTAGACTACCTAGACTACCTAGACTACCTAGACTACCTA44830.12000103859921875No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC40820.10926706213741043No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT12750.028.5509474
CTAGCGC8350.025.1493835
TATACTC12650.024.349475
TCCTATA10950.023.0152512
CGAGACT98150.022.53664470
GACCTAT11350.021.904841
GTGCGTT9650.021.7613826
CTCCTAT11150.021.6696451
TACTCCG11850.021.265557
GCGACAT10300.021.06769219
TAGAGTG9100.020.7706345
TAGCGCC10700.020.60721436
ACGAGAC139750.020.51136670
CCGTTGC11650.020.42892314
CCTATAT11050.019.9560993
ACTCCGC14700.019.7616528
GTTGCGA11550.019.69671616
CACGAGA198050.019.59833570
CCTATAC18750.019.4146423
TTGCGAC11750.019.36145417
Produced by FastQC (version 0.11.5)