FastQCFastQC Report
Thu 24 Dec 2020
HK3T5AFX2_n01_AA01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n01_AA01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1518.29268292682927No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC22.4390243902439024No Hit
CGATAAACCGGATGGATCGAAAAAGACAGTGAGGAGGAGAAGGAAGGCGG11.2195121951219512No Hit
GATGAAGGGAGGGAGCATCGCGCCGTGCTACAACAAGTTCCGGAAGGGGT11.2195121951219512No Hit
GGTGGAGTCAGCGGGTTTTTCCTATGCCGAAAGGTATCGGTAAACCGTTG11.2195121951219512No Hit
GAATGATGGAACGACCAACAAAAAAAACAAACACCGTACCTGCTCTTATA11.2195121951219512No Hit
AGCCAATCCTTTTCCCGAAGTTACGGATCTAGTTTGCCGTCTTCGGGTTG11.2195121951219512No Hit
CTCCAAAACCGCTCCATAACCGCTCCAAAACCGCTCCAAAACCGCTCCAT11.2195121951219512No Hit
AATCCAGCCTTTCACTATTAATGCCCGTATTAACGGACATTAAAATCAGG11.2195121951219512No Hit
CGCGAGCCGCCAGCGAAGACCTAGAAGCACTAGCATCCAGATCAGCACAC11.2195121951219512No Hit
GGTGAGTTGGGCGGCTCAATGAGCAGCGTTGCACAGCCGACGCCCAAACG11.2195121951219512No Hit
GTCGACGCCCTTGTCGTCCAGGCCGTTCCAGGAGCAACTACTGCGGGCGG11.2195121951219512No Hit
CTTTCTCTGTGCTCTCTCATTTCTTTATGATCTATGAGAAGTGATCAGCG11.2195121951219512No Hit
GCTATAGGTGAACTTCTGGAGTCTCATAAAATCGAGGCTCAGTTACTCTA11.2195121951219512No Hit
GTTAAGTTTTGCTCGGATGGTTATCTTTGACCTGAGTAAGTCCGTGTGCA11.2195121951219512No Hit
AAATATAGTGATCAGCTGATGCTATTCGTATTAGCTTATCTTATGCTACA11.2195121951219512No Hit
ACATTAGGTTGAGCTCAACTGTCCAACCCCGTCAAACTTTTTCCACTTTT11.2195121951219512No Hit
GCCGACCTGTGGCACACCTCTGCGGCGACTCCAGAGACAGCGGTGCCTCT11.2195121951219512No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGG11.2195121951219512No Hit
CTCATGCTCACAGTAGGCATCTAAAGCTCATTGGTGATGTCGTGGTGTGT11.2195121951219512No Hit
CACATCTCCGAGCCCACGAGACAGCAGAAATCTCGTATGCCGTCTTCTGC11.2195121951219512RNA PCR Primer, Index 17 (96% over 25bp)
TATATAATGAGTGCTTATCACCTCCGTTGTTGTCTAGTGGTTAGGATTTA11.2195121951219512No Hit
GCCTCAGGACATGCTCAAACGTGTCAAGCCTGCCGAGAAGAAGGAGAAGA11.2195121951219512No Hit
CTATTTAACGATCAATGTAAATTATAGAAAAGCCAGAGAAAAAAGTAGAG11.2195121951219512No Hit
GATATCGACCAATTAAAGATCTAAACAGTATTGTTACTGATTATTTCAGG11.2195121951219512No Hit
GTACAACATAGTATGCGCTCCGCCTCGCCATATACATCTGCTTCGATTAA11.2195121951219512No Hit
CTTCCAATATCCGCCCGCTATTCTTCTGTGTGCTCAGTCAAAAGACAACG11.2195121951219512No Hit
GTAGTACAGTTCTGTCTATGTCTCTACTATGCAACCCGTCGTCTCGCGTT11.2195121951219512No Hit
CATCTAAACTACTTGTCCGCTACCTTATGTACCTCATTGACTCATTTTGC11.2195121951219512No Hit
GTCCCATACTCCGTAGTCGTAAAGTCCAACAACTCGACGAACACCGTTTG11.2195121951219512No Hit
CTGCCGAACGCCTCATCTTCGAGGCGGGAAGGCGCGACGCGGTCCCCCAA11.2195121951219512No Hit
GTGTTTCAGCGTATACCACCAGTTAAGAATAGTATTATCTAATAATATTT11.2195121951219512No Hit
GTGTAATCCGACGGCGGCGCGCGCGGTTCAGTATCTGTCTCTTATACACA11.2195121951219512No Hit
AACACTTACAAACTTATCAAGAAATTAACTTTGTGGTAAACCTTTGTCAT11.2195121951219512No Hit
GTGTACTCCACACGGACAAATACATTTAATTTTACAACTAAAATCGAGCC11.2195121951219512No Hit
CCTATATGATGGGCTCGACGGCAACGCTCAATGCTCGTCGGCAGTACAGG11.2195121951219512No Hit
ACCCAATATGTTTCACTTGTATTGTAGAAAAAAATGAAGAAGAATATAAC11.2195121951219512No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGGCGTCTTCTGCT11.2195121951219512Illumina PCR Primer Index 4 (95% over 24bp)
GTGCAAGTTGGCTCGAGGCTCCAACGTTCTCATGTTAACCAGTTACGGTT11.2195121951219512No Hit
CCGCATGACGTTCTGCCCCATCAGAGAAGAACACGAACTTGTAATAATCC11.2195121951219512No Hit
GTTCAGGGTAGAAATAGTAAAAATCAAGAGGGATGTACGAGGTCTGGCGA11.2195121951219512No Hit
GTCTTTCGACACAAGCACTGAATGCAGTTGTGGTTGTGCTTGAGTACTAA11.2195121951219512No Hit
AGTTATTGGATAAAAAAAGGTGGTTGTTACGCAAAAGAAATACTAAAATA11.2195121951219512No Hit
GAATGCACCCTAACGTGCAACACACAACGGCGGTGGAAGAATGGTGTAGA11.2195121951219512No Hit
GGCGTCGTTCGCCGTTCATCGTAACCAGGCACTTTCAGTTAAGCACCACC11.2195121951219512No Hit
CTTTTAATTGTTGCAACCCATTTCAAATTGGCGGTTAACTGAAATTGAAA11.2195121951219512No Hit
TCTTTGAACAAAAAAACAACAAGGAAGTAAGTAAAGAGTGAAAAATTACA11.2195121951219512No Hit
AGAGTGATGTCTTAAAGATTCTGCTCTACGAACCAGGGGAAACTGCAGGG11.2195121951219512No Hit
ATCGAACTCAGTGGCCATCATTTTCCTGCCATTTGAAAGGAGGACATTAT11.2195121951219512No Hit
TTTTGGTAATGGGTCAGAACATAGGAGAGTAGATTGGATCGCGAAGCAGA11.2195121951219512No Hit
CTCCTAGCGTGCAAGGCTACTAAAAACCAGTTAATTCACTCGGCAGAGCT11.2195121951219512No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGG11.2195121951219512No Hit
CCATTAACCAGCATTTTAGGATCCTGACTGTTAATACCTGCTTGTCGGTC11.2195121951219512No Hit
AAATAACGCTATAAAAACACATAAACACAGTCAAAACGATACGATAGCAA11.2195121951219512No Hit
GTTCACTACTGTTTATCCTTCGTATAGTGGGTGGACTCGTCAGATAATTA11.2195121951219512No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGG11.2195121951219512No Hit
CGACCTATGTTTATTGCTCCCTAGGGTCGCAATAATAATTAATAACACGC11.2195121951219512No Hit
TGTGTACACAGGTCAGTTGCCCAAAGGCCCACACAACATTGCTCAGGTCA11.2195121951219512No Hit
GATTCCTTGTATGGTTCGTGTGGTACTGGGGGTTTTGGTGAGCGGTGGTT11.2195121951219512No Hit
GGATGACAAAACCCGCAAGACGTCTGCCGCCTCTTGTGTCCCCCCTATAT11.2195121951219512No Hit
TAAATCGAAGTGGACTGCTGGCGGAAAATGAGAAAATTCGACCTATACTT11.2195121951219512No Hit
GAATGGCACACACCTGTAATACATCTATAATCGCAAATTGGAAATTTTTT11.2195121951219512No Hit
GGCGGGGACGCCGCCCCTTTAAGTTATGGTACTTCTTAAACACTGGGACG11.2195121951219512No Hit
ACCCTCGCTTTCCTGCTCCTGTTGAGTTTATTGCTGCCGTCATTGCTTAT11.2195121951219512No Hit
CCCAGAAGCCTTCGCACCAGGCTTTGCAGACCGACTTCAGGACCTGTCTC11.2195121951219512No Hit
ATTTAGGGCGAAAACATTCAAATAAACGAAAAATTCACATTAAACGCCGT11.2195121951219512No Hit
ACTGTGCAGAGCTTTAAAGTTGAATGCAACTTTGGCCGCCTCGTTCCAAA11.2195121951219512No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTGA50.070.026
GATGGAT50.070.011
GGAGAGA50.070.061
GGATGGA50.070.010
GGAGAAG50.070.036
CCGGATG50.070.08
GAGAAGG50.070.037
ACCGGAT50.070.07
AAACCGG50.070.05
CCAACCT50.070.068
AACCGGA50.070.06
TAAACCG50.070.04
CGATAAA50.070.01
GAGGAGG50.070.031
GAGGAGA50.070.034
GACAGTG50.070.025
AGAAGGA50.070.038
CAACCTA50.070.069
TCGAAAA50.070.017
GAAAAAG50.070.019