FastQCFastQC Report
Tue 5 Jan 2021
HK3T5AFX2_n01_AA01_.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3T5AFX2_n01_AA01_.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7378330
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG126060.17085166968677193No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC90410.12253450306505673No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC157250.022.5018670
CACGAGA218100.019.81842870
CGAGACG104050.018.43301670
CCACGAG287400.018.29115370
CTATACT17700.015.821524
CCCACGA338750.015.46679869
TATACTC19300.015.2353865
GCCCACG382200.013.69931468
TTATACT25700.013.4844614
GAGACGG78600.013.40299470
ACCACGT30600.013.1532888
AGCCCAC410550.012.75332567
TAATACT29150.012.7291334
TACCGGC23200.012.67211852
TACACCG17450.012.6379485
GGACTGC12800.012.5799636
ATACCGG23800.012.49970851
TATACCG12600.012.2240085
TACACTG34150.012.1979755
ACCGTGT13550.012.1399388
Produced by FastQC (version 0.11.5)