FastQCFastQC Report
Fri 11 Aug 2023
HK3JJBGXT_n01_daplib_ShaAmpPoolHm-input_ad4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3JJBGXT_n01_daplib_ShaAmpPoolHm-input_ad4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1532
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGATCGGGTTGCGGTTTAAGTTCTTATACTCAATCATACACATGACATC30.19582245430809397No Hit
AAAACAGAAAAAAAAAACAGAAAGCAGTATATCTTGAATTATCAAAATTT20.13054830287206268No Hit
ACACATGACATCAAGTCATATTCGACTCCAAAACACTAACCAACCTTCTT20.13054830287206268No Hit
GACTGAAAATCCTTCTGTCTGCGGTTCGAATCCGGACTCACTTCTAGCTC20.13054830287206268No Hit
TCATACACATGACATCAAGTCATATTAGACTCCAAAACACTAACCAACCT20.13054830287206268No Hit
TACAAAGCCAAAGACTCATACGGACTTTGGCTACACCATGAAAGCTTTGA20.13054830287206268No Hit
AGCAAAGTAGCTAGGCTTCAGCTCATAATCGTTCCTCTAAAAGGGTGAGA20.13054830287206268No Hit
ATGGACTTTGGCTACACCATGAAAGCTTTGAGAAGCAAGAAGAAGGTTGG20.13054830287206268No Hit
TTCTCAAAGCTTTCATGGTGTAGCCAAAGTCCATATGAGTCTTTGGCTTT20.13054830287206268No Hit
TTTAAGATCGGGTTGCGGTTTAAGTTCTTATACTCAATCATACACATGAC20.13054830287206268No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)