FastQCFastQC Report
Fri 11 Aug 2023
HK3JJBGXT_n01_daplib_ColAmpPoolHm-input_ad4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3JJBGXT_n01_daplib_ColAmpPoolHm-input_ad4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3385
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATGCC220.6499261447562776TruSeq Adapter, Index 7 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTAATGATCTCGTATGC140.413589364844904TruSeq Adapter, Index 7 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)