FastQCFastQC Report
Fri 11 Aug 2023
HK3JJBGXT_n01_AtbZIP4_ampCol-B_ad2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3JJBGXT_n01_AtbZIP4_ampCol-B_ad2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5689098
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATGCC91370.16060542462091532TruSeq Adapter, Index 7 (96% over 32bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGGGGTTGGC59930.10534183098972807TruSeq Adapter, Index 7 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)