FastQCFastQC Report
Fri 11 Aug 2023
HK3JJBGXT_n01_AtbZIP28_ampCol-B_ad2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3JJBGXT_n01_AtbZIP28_ampCol-B_ad2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12976538
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATGCC3024112.3304443758420006TruSeq Adapter, Index 7 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT268600.20698895190689537TruSeq Adapter, Index 7 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGAGCTCGTATGCC156950.12094905436257344TruSeq Adapter, Index 7 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)