FastQCFastQC Report
Fri 11 Aug 2023
HK3JJBGXT_n01_AtbZIP26_ampCol-B_ad2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3JJBGXT_n01_AtbZIP26_ampCol-B_ad2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19505859
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGGGGGTGGC308220.15801406131357765TruSeq Adapter, Index 3 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGGGGGTGGG296290.15189795025176794TruSeq Adapter, Index 3 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGGGGTTGGC285320.1462739990071701TruSeq Adapter, Index 3 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGGGGGGGGG222760.11420158425219827TruSeq Adapter, Index 3 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGGGGTTTGC195110.10002635618354463TruSeq Adapter, Index 3 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)