FastQCFastQC Report
Fri 11 Aug 2023
HK3JJBGXT_n01_AtbZIP26_ColAmpPoolHm-B_ad6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3JJBGXT_n01_AtbZIP26_ColAmpPoolHm-B_ad6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20489
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTTACATATGCTGATATGCATGCATATGTCATGTGTGCATGGAGTGA400.19522670701351943No Hit
AATCGCGGCGCGGAAAACGGTGCCGTTGAATATAACGTTGACGCTAATGG350.17082336863682954No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATGCC220.10737468885743569TruSeq Adapter, Index 7 (96% over 33bp)
CCTTCATCGTCTCCAAAATCTATATCTCGTCCTGACCAATCAGAAGATAT210.10249402118209772No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTAATGATCTCGTATGC210.10249402118209772TruSeq Adapter, Index 7 (96% over 33bp)
GACGGTGGCGAGAACGATTCTTACGTCAACCGTACGACGGTGACGACGGC210.10249402118209772No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)