FastQCFastQC Report
Fri 11 Aug 2023
HK3JJBGXT_n01_AtbZIP21_ampCol-B_ad1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3JJBGXT_n01_AtbZIP21_ampCol-B_ad1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6186592
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC1329152.1484364897507384TruSeq Adapter, Index 2 (97% over 34bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTTTGCC150450.24318720225933763TruSeq Adapter, Index 2 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT142400.23017519176955584TruSeq Adapter, Index 2 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGGC134010.21661360568144788TruSeq Adapter, Index 2 (97% over 34bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATTCC95630.15457621902333304TruSeq Adapter, Index 2 (97% over 34bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTTTGGC77840.12582048404032464TruSeq Adapter, Index 2 (97% over 34bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCGCGTATGCC71200.11508759588477792TruSeq Adapter, Index 2 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)