FastQCFastQC Report
Fri 11 Aug 2023
HK3JJBGXT_n01_AtbZIP19_ampCol-B_ad2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3JJBGXT_n01_AtbZIP19_ampCol-B_ad2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18826253
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGGGGGTGGC435870.2315224383736902TruSeq Adapter, Index 3 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGGGGGTGGG405460.21536946305778423TruSeq Adapter, Index 3 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGGGGTTGGC399860.21239489345011991TruSeq Adapter, Index 3 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGGGGGGGGG298550.15858174220860624TruSeq Adapter, Index 3 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGGGGTTTGC272090.14452690081239214TruSeq Adapter, Index 3 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGGGGGTTGC252560.13415308930566266TruSeq Adapter, Index 3 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGGGGGTTGG215850.11465372318113436TruSeq Adapter, Index 3 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)