FastQCFastQC Report
Fri 11 Aug 2023
HK3JJBGXT_n01_AtbZIP15_ampCol-B_ad2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3JJBGXT_n01_AtbZIP15_ampCol-B_ad2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6531953
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATGCC1191001.823344411694328TruSeq Adapter, Index 19 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT124150.1900656664247278TruSeq Adapter, Index 19 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTTTGCC120870.18504419734802133TruSeq Adapter, Index 19 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATGGC114100.17467976269884367TruSeq Adapter, Index 19 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATTCC89870.1375851908303688TruSeq Adapter, Index 19 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCGCGTATGCC73320.11224820509271882TruSeq Adapter, Index 19 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTTTGGC66250.10142448973530581TruSeq Adapter, Index 19 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)