FastQCFastQC Report
Fri 11 Aug 2023
HK3JJBGXT_n01_AtbZIP15_ampCol-B_ad1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3JJBGXT_n01_AtbZIP15_ampCol-B_ad1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4573863
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGGGGG765791.6742740217623484TruSeq Adapter, Index 6 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGTGGG78350.17129940271494795TruSeq Adapter, Index 6 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG77050.16845716629466165TruSeq Adapter, Index 6 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)