FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_H10_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_H10_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences778352
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA14370.18462083992846426No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG11220.14415071844101382No Hit
ACCCTGGACCCTGCATAAGATGCAATAAACATGGCTACAGTCATGTTAAG11160.14337985898411001No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT11110.14273747610335683No Hit
CTCTCTCACCCCACCTTCCTCTAGCATGCACCTGGCTGCCTTTGCCTCCC10480.13464345180586673No Hit
AAACATGGCTACAGTCATGTTAAGGGCAGAGGCTCACTGGGGTAAGTGGC9840.12642095093222605No Hit
TCCCACCTGCAGTCTGGATTCTGTCCCAATCACACACTAAAGAAGAGCCC9750.12526466174687031No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA9160.11768454375398277No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC9050.11627130141632577No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC8900.11434415277406625No Hit
GTCACGCAGTCGGCTCTGGTTCTCCTCCCACCTCTCTGACCATTCCTTCT8180.10509383929122043No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)