FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_H08_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_H08_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1102291
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC61280.555933052161362No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA36980.3354830983832763No Hit
CTCCTGGACCAGTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCA28360.25728233288668784No Hit
AGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCG22830.20711409237669542No Hit
GGAACAGAGAGCCATTGCTAAGATCAAGATGTTGGGAAACATCAAATTCA19350.17554348171217946No Hit
GTCCTTAATGGATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTA18960.17200539603425957No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATCATTGTCAGGGCC18120.16438490380489362No Hit
CCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGATCGTCTTTGGT17810.16157257929167523No Hit
GCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT17150.15558504968288775No Hit
GGCTAATCTCATCTGCATTAAGCTGTCCTTTCTTAGAAAACCGAGGTGGC16640.15095832225791556No Hit
GGAATAAACACCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGAT16180.14678519556088182No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT16160.14660375526970645No Hit
GTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCAGCAAGTCCTCC16130.1463315948329434No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC16100.14605943439618033No Hit
CTTCATAAGTGCATCAAAACACTTTTGGAAAAGAAGAAGAGAGTCCAACT16000.1451522329403034No Hit
TCCTTCGACTGTCCTTGCAGCTGGGATAAGAGTCCCTGACTCTGGTTATG14330.13000196862715926No Hit
TCATTGGATTGTGCAAACGCATCAAATGTTCAGGCTGTGAACCGTTCTGC13890.12601028222130092No Hit
CCATTATACTTGACATACAGTCCCATCCCCATCCTTTCAGTCTTAGACAT13840.12555668149336247No Hit
GATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTAAGGAATTGCC13810.1252845210565994No Hit
ATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCACTGTCCTCAT13360.12120211450515335No Hit
GGAATGGTATGTGTCAGTATATGCTTCTCCATATTTTACTTTGAGCAATG13040.11829906984634729No Hit
CATATCAGCAAGTCCTCCAAGTGGGTCCCTATCCATTTTCATCCTGGGTG12910.11711970795370732No Hit
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG12900.11702898780811964No Hit
GTTTATTCCTGCTCCTATGGCTCAAGGGATGAGAAGTGACTTCTTTCTGG12680.11503314460519047No Hit
GTCCCATGGTGGGTGAAAATCTATCCTGGATAACTCCTGGACCAGTACCA12170.11040641718021828No Hit
GTCTATGATAAGCGTGAAAATCCCCTCCTCCCCGAGGAGGAGGAACAGAG12110.10986209630669216No Hit
GAAGAAGAGAGTCCAACTCAAAGATATGGGAGAGGATTTGGAGTGCCTCT12050.10931777543316601No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT11920.10813841354052604No Hit
GATTTGGAGTGCCTCTGTCAGATAATGAGGACAGTGGGACCTAGATTAGA11840.10741265237582452No Hit
GATCAAGATGTTGGGAAACATCAAATTCATTGGAGAGCTTGGCAAGCTTG11770.1067776113567107No Hit
AGCAGGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC11480.1041467271346677No Hit
TCTTTGGTCCATTGTCAAGAAAAGCCTTGCGAGGAACCCAATGGTGTTCT11350.10296736524202774No Hit
ATCTAGGGGTGTTTATTCCTGCTCCTATGGCTCAAGGGATGAGAAGTGAC11300.10251376451408929No Hit
CATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAATTTGAAAACCGA11130.10097152203909857No Hit
CCATTGGGTTCCTCGCAAGGCTTTTCTTGACAATGGACCAAAGACGATCA11050.10024576087439706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)