FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_H08_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_H08_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911013
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC26000.28539658599822393No Hit
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA25000.2744197942290615No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA24430.2681630229206389No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC24430.2681630229206389No Hit
TCCCACCTGCAGTCTGGATTCTGTCCCAATCACACACTAAAGAAGAGCCC19710.21635256577019207No Hit
ACCCTGGACCCTGCATAAGATGCAATAAACATGGCTACAGTCATGTTAAG18910.2075711323548621No Hit
AAACATGGCTACAGTCATGTTAAGGGCAGAGGCTCACTGGGGTAAGTGGC18290.2007655214579814No Hit
GGCCAGGCGAGGTGACTCACGCCAGCAATCCCAGCACTTTGGGAGGCCGA16470.1807877604381057No Hit
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA15930.17486029288275798No Hit
ATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCACACAAGT15530.17046957617509298No Hit
CCACACTGGCAAACCCTAAGGCCCCTTGCCAGTGCTCACCTTGCGTGGCC14630.1605904635828468No Hit
GAGTGAGACCCTGTCTCAAAAACAAGAAAGCTAAATTTGTGAACAGTTAA14230.15619974687518182No Hit
CTTCTTTAGTGTGTGATTGGGACAGAATCCAGACTGCAGGTGGGATGTGA14060.1543336922744242No Hit
GTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCCTAGCT14020.1538946206036577No Hit
GTCCAGGGTCTAGAGAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGG13880.15235786975597493No Hit
CTCAAAAGGCTGAGGCAAGAGAGTCACTTGAGTATGGGAGGTGGGGGCTG13870.15224810183828333No Hit
CTCTCTCACCCCACCTTCCTCTAGCATGCACCTGGCTGCCTTTGCCTCCC13720.15060158307290897No Hit
CTGCACTCCATCCTAGGAGACAGAGTGAGACCCTGTCTCAAAAACAAGAA13550.14873552847215135No Hit
ACACGATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA13450.14763784929523507No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC13410.1471987776244686No Hit
GTACCACACTGGGTGCTGAGACTTGGCCTTCTTCACACAAGTCCTCCTGT13320.14621086636524397No Hit
TCTTTAGTGTGTGATTGGGACAGAATCCAGACTGCAGGTGGGATGTGAGG13170.14456434759986958No Hit
ACACAATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA13050.1432471325875701No Hit
CTAGAAAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGGCAGCCAGGT12600.138307576291447No Hit
GCAGGCAGGATGTGAGGTTGCTCCCTCTCCACCTGCTTCAGCCCTGCCAC12460.13677082544376423No Hit
GTATATGAGGGTGTCTGAGGATTGTAAAATTATCAGGAAATCCCTCTTGG12300.13501453876069827No Hit
GAGTAGGGGAGGGCCAGAGACAGAGAGGAGACAGCAGCTAGAGCACCCGT12110.1329289483245574No Hit
CTTTCTCTAGACCCTGGACCCTGCATAAGATGCAATAAACATGGCTACAG12020.13194103706533278No Hit
GGATTGCAGTGGTACGATCTCAGCTCACTGCAGCCCCCACCTCCCATACT11230.12326937156769444No Hit
CTATAAAAGTGAGGTGCTGGCAGGACGTACTAGCAGGCACGTGGGATGCT11190.12283029989692791No Hit
GCACATGCCTGTAGTCCCAGCTACTCAAGAGGCTGAGGCAAGAGAGTCAC11090.12173262072001167No Hit
GAACCAGTTAAGAGGCTGTTTTTGATCTGGGACAGAGAGAGGGTGATGAC10870.11931772653079593No Hit
GAGCAAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAAGAGGC10770.1182200473538797No Hit
CACTTATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTG10590.11624422483543044No Hit
CGATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGG10500.11525631357620582No Hit
CTCTCCACCTGCTTCAGCCCTGCCACTTACCCCAGTGAGCCTCTGCCCTT10410.11426840231698121No Hit
ATCCACAAGTATCTTAACTGTTCACAAATTTAGCTTTCTTGTTTTTGAGA10350.11360979481083144No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC10350.11360979481083144No Hit
CTAAAGAAGAGCCCTTGTCAAGGTTACCAGCAACCTCCACATTGGCAAAC10330.1133902589754482No Hit
CACAAACAGCCTCTCTCACCCCACCTTCCTCTAGCATGCACCTGGCTGCC10170.11163397229238221No Hit
CTTATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAA10140.11130466853930734No Hit
CGTCCTAATAACAAGGGACCAATACATGGGTACCACACTGGGTGCTGAGA10070.11053629311546596No Hit
TTGCATGCCCCTCTTCATTCCTCAGGGCTCTGTCCTCAAACCATCCTTTT10060.11042652519777435No Hit
GAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGCACATGTTTGA10000.1097679176916246No Hit
GTTAAGGGCAGAGGCTCACTGGGGTAAGTGGCAGGGCTGAAGCAGGTGGA9910.10878000643239998No Hit
GTATCTTAACTGTTCACAAATTTAGCTTTCTTGTTTTTGAGACAGGGTCT9750.10702371974933397No Hit
GTCACGCAGTCGGCTCTGGTTCTCCTCCCACCTCTCTGACCATTCCTTCT9740.10691395183164236No Hit
TCCTACAGCACCCTGGAATGGCTGAAATTAAGTATTTTATATGGCAAAAT9590.105267433066268No Hit
GTCATGTTAAGGGCAGAGGCTCACTGGGGTAAGTGGCAGGGCTGAAGCAG9140.10032787677014489No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)