FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_H08_FluA_Rep1_Plate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_H08_FluA_Rep1_Plate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657868
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC37170.22420361572815206No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA36610.22082578347612716No Hit
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA34570.20852082312946507No Hit
AAACATGGCTACAGTCATGTTAAGGGCAGAGGCTCACTGGGGTAAGTGGC34540.20833986783024946No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC33530.20224770608999026No Hit
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA29570.17836160659352854No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC28420.17142498679026316No Hit
CCACACTGGCAAACCCTAAGGCCCCTTGCCAGTGCTCACCTTGCGTGGCC26960.1626184955617697No Hit
TCTTTAGTGTGTGATTGGGACAGAATCCAGACTGCAGGTGGGATGTGAGG26920.1623772218294822No Hit
GTATATGAGGGTGTCTGAGGATTGTAAAATTATCAGGAAATCCCTCTTGG26290.1585771605459542No Hit
ATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCACACAAGT24350.14687538453001084No Hit
TCCCACCTGCAGTCTGGATTCTGTCCCAATCACACACTAAAGAAGAGCCC22400.1351132900809956No Hit
CTTCTTTAGTGTGTGATTGGGACAGAATCCAGACTGCAGGTGGGATGTGA21820.13161482096282698No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC20280.12232578226975852No Hit
TCGTAGGCTTTGGAGCTGTGTTTGAATCCTAGCTCTGTTATCTTCTAGCT19880.1199130449468836No Hit
GGCCAGGCGAGGTGACTCACGCCAGCAATCCCAGCACTTTGGGAGGCCGA19710.11888763158466174No Hit
GTCCAGGGTCTAGAGAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGG19690.11876699471851801No Hit
GTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCCTAGCT19600.11822412882087113No Hit
ACCCTGGACCCTGCATAAGATGCAATAAACATGGCTACAGTCATGTTAAG19490.11756062605708054No Hit
GTTAAGGGCAGAGGCTCACTGGGGTAAGTGGCAGGGCTGAAGCAGGTGGA18920.11412247537198378No Hit
CTCAAAAGGCTGAGGCAAGAGAGTCACTTGAGTATGGGAGGTGGGGGCTG18880.11388120163969628No Hit
ACACGATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA18840.1136399279074088No Hit
GAGTGAGACCCTGTCTCAAAAACAAGAAAGCTAAATTTGTGAACAGTTAA18750.11309706200976194No Hit
ATCCACAAGTATCTTAACTGTTCACAAATTTAGCTTTCTTGTTTTTGAGA17770.10718585556871837No Hit
GAGCAAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAAGAGGC17690.1067033081041434No Hit
CTAAAGAAGAGCCCTTGTCAAGGTTACCAGCAACCTCCACATTGGCAAAC16820.10145560442689043No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)