FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_H06_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_H06_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1500429
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC92050.6134912081811269No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC36130.24079779849629673No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTGTAGTAAGGACAATT34700.23126719091673115No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT33080.22047027883358694No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC32550.21693795574465702No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT28160.1876796569514452No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT27460.1830143245698397No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTATTGTCAGGACC27410.18268108654258217No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA25690.17121769840492285No Hit
AGCATATACTGACACATATCATTCCTATGCAAACAACATCCTAAGAACAC25530.17015133671769875No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT24810.1653527091251902No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG24800.16528606151973868No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA24140.1608873195599392No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA23850.15895453900184547No Hit
TATCATTCCTATGCAAACAACATCCTAAGAACACAAGAAAGTGCCTGCAA22200.14795768410234672No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA19560.13036271626314874No Hit
GTTCTTTATTATTGTCATCCCTATTGCCATATCTGCTGATTCATTTACTC19460.12969624020863366No Hit
CCCCCAGACCAGATGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG19310.12869652612686105No Hit
GCACTATACCTTTTCCTTTTGACTACTTTAACTGGTTTTCCTTGATAAAG18850.12563073627609173No Hit
ATACTGAGGAGTGCACATGCGGATTTGCCAGCAATAAAACCATAGAATGT18470.1230981272689344No Hit
AACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACA18260.12169852755445276No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC18000.11996568981271358No Hit
GCTGTAGAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCA17170.11443393856023842No Hit
CAATAATGCATTGCTCAAAATAAAATATGGAGAAGCATATACTGACACAT16550.11030178702224497No Hit
ATCATGTACCATCTCTATCCCAATACAGGGGACATCGCATTTCTTATCTT16110.10736929238237863No Hit
TTATAGAGCCATGTCGACACCTGTGACTGTGTCCCACAGCAGCTGTCCTG15600.10397026450435175No Hit
TCATAGATGTGCCCATACAGGCAGCAATTTCAACAACATTCCCATACACT15550.10363702647709422No Hit
GCATTATTGTCAGGACCATCAACTCCGATATATGTCCATTCCTTACCATC15470.10310384563348217No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)