FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_H05_FluA_Rep1_Plate3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_H05_FluA_Rep1_Plate3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1634510
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCAGGTGTTTATC95030.58139748303773No Hit
GTCCTGGAGGGATTCATAGGAGAGGCACATTTCAGGCCAAGAGAGTTTCA86650.530128295330099No Hit
GATATGGGGTTCCTCTGCCCAATAAGCAGATGTTCTGGTGGTGAAGACGA82310.5035759952523998No Hit
ATTCTGGACAGCTGGATTGGTGAAGATGAAGGTCTGCTGAGCAAGGCAGG53980.3302518797682486No Hit
GTTTAGAAGTGGGTTCGTATAGAAAAAAACATCCTGAAGAAAGACAGTTT53550.32762112192644893No Hit
CCTGTAAGACCATAAACACTGGAGATGGGGAATTATGAAATGGTATGAGT52730.32260432790255184No Hit
TCCCCACACAGGCCACAAGGTGGGAAGTAGGAGATAATGACTCCCACTTG50880.3112859511413206No Hit
CTCCAGGACAGTCATCCACCTCAGGTAGAAAGTTTCTGCCCTTTCCCCAC49210.3010688218487498No Hit
GATATGAAGATCACTGAGGCATCTGTGCAGCCAAGGCAGGTAGAGCAAAA41070.2512679640993325No Hit
ATTCATAGGAGAGGCACATTTCAGGCCAAGAGAGTTTCATGCTTAACACC40630.24857602584260727No Hit
GTTTTAAGTACCGCTGATTTTCTTGCAGCTATTTGCCTAATTCCTCTGAC40410.24723005671424464No Hit
GAGCAAAAGCAGGCCATTCTGGACAGCTGGATTGGTGAAGATGAAGGTCT39090.23915424194406887No Hit
ACCATAAACACTGGAGATGGGGAATTATGAAATGGTATGAGTGGGGAAAG38940.23823653572018527No Hit
GAATAAAAGAGGGATAAGATATGGGGTTCCTCTGCCCAATAAGCAGATGT37880.23175141173807443No Hit
GCCTAGTAGTAGGAATAAAAGAGGGATAAGATATGGGGTTCCTCTGCCCA37470.22924301472612585No Hit
TTGCTAGTTAGTTCGAGAATACTACCAGGTGTTTATCACCAGCACCAATT36660.2242874011171544No Hit
GATTATACTGTCTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCAG36300.22208490617983373No Hit
GCATTAGGCTGAGGGATGGGCCTAGTAGTAGGAATAAAAGAGGGATAAGA35890.21957650916788518No Hit
GTCTAAGCTCACACAGCCAGGTGGAGAGGAGCAGAGCTGAGATTCAAGCC35500.21719047298578778No Hit
TGATTATACTGTCTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCA34520.21119479232308155No Hit
GATAAATACCTTACTTAGTTCAGTTTATACAGCCTTGTTAGCAATCTGAG33820.20691216327829134No Hit
ATTATACTGTCTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCAGG32740.20030467846632935No Hit
GGCTAAGACAGGAACAGGCAGGACACAATTGTTACTTGACGGCCCTGCCT32320.19773510103945524No Hit
CTTAAAGACAGTATAATCACACCAAGCTCCTAGTAAATATCCCATGATGG32300.19761274020960412No Hit
GTAATTACCATCATGGGATATTTACTAGGAGCTTGGTGTGATTATACTGT32250.19730683813497624No Hit
CCTATGAATCCCTCCAGGACAGTCATCCACCTCAGGTAGAAAGTTTCTGC30890.18898630170509817No Hit
CTTCTGTAATTACCATCATGGGATATTTACTAGGAGCTTGGTGTGATTAT30470.18641672427822406No Hit
GGTATGAGTGGGGAAAGGGCAGAAACTTTCTACCTGAGGTGGATGACTGT30140.1843977705856801No Hit
GGATAAATACCTTACTTAGTTCAGTTTATACAGCCTTGTTAGCAATCTGA29990.1834800643617965No Hit
AGTTTATACAGCCTTGTTAGCAATCTGAGCTCAACTTCCAGGCCAGCCTC29700.18170583232895487No Hit
CCACAGAGATATGAAGATCACTGAGGCATCTGTGCAGCCAAGGCAGGTAG29420.17999278071103877No Hit
TCATATCTCTGTGGTGTTAAGCATGAAACTCTCTTGGCCTGAAATGTGCC28720.17571015166624862No Hit
GAGATATGAAGATCACTGAGGCATCTGTGCAGCCAAGGCAGGTAGAGCAA28720.17571015166624862No Hit
GTTTATACAGCCTTGTTAGCAATCTGAGCTCAACTTCCAGGCCAGCCTCC28090.17185578552593742No Hit
GGATAAGATATGGGGTTCCTCTGCCCAATAAGCAGATGTTCTGGTGGTGA27650.1691638472692122No Hit
GGCCACAAGGTGGGAAGTAGGAGATAATGACTCCCACTTGTGTATTGATA27480.16812378021547741No Hit
ACCTTACTTAGTTCAGTTTATACAGCCTTGTTAGCAATCTGAGCTCAACT27370.1674507956512961No Hit
GTTTATCACCAGCACCAATTCACAAGGATGAGCCTGAGGCTCAGAATGGT26960.16494239863934756No Hit
GTGGATGACTGTCCTGGAGGGATTCATAGGAGAGGCACATTTCAGGCCAA26680.1632293470214315No Hit
GCATTCACCTGTAAGACCATAAACACTGGAGATGGGGAATTATGAAATGG25560.1563771405497672No Hit
CTTCATATCTCTGTGGTGTTAAGCATGAAACTCTCTTGGCCTGAAATGTG25160.15392992395274424No Hit
GTATAATCACACCAAGCTCCTAGTAAATATCCCATGATGGTAATTACAGA24890.15227805274975376No Hit
GGATGAGCCTGAGGCTCAGAATGGTGAAGCACCTTGTCTAAGCTCACACA24490.1498308361527308No Hit
TTTACTAGGAGCTTGGTGTGATTATACTGTCTTTAAGTACGTTGCTAGTT24090.1473836195557078No Hit
ACTTAGTTCAGTTTATACAGCCTTGTTAGCAATCTGAGCTCAACTTCCAG23810.14567056793779176No Hit
GAATGGTGAAGCACCTTGTCTAAGCTCACACAGCCAGGTGGAGAGGAGCA23700.14499758337361043No Hit
CTTGTGTATTGATAGGATAAATACCTTACTTAGTTCAGTTTATACAGCCT23690.14493640295868487No Hit
GCTCCATACCCAGCACATAAACTTAATATTATTCCCCACACAGGCCACAA23580.14426341839450355No Hit
GTGAAGCACCTTGTCTAAGCTCACACAGCCAGGTGGAGAGGAGCAGAGCT23480.1436516142452478No Hit
GTGTTTATCACCAGCACCAATTCACAAGGATGAGCCTGAGGCTCAGAATG23300.14255036677658747No Hit
ACTTAATATTATTCCCCACACAGGCCACAAGGTGGGAAGTAGGAGATAAT22100.1352087169855186No Hit
CTTAAGGGGAGGCAGGCATTAGGCTGAGGGATGGGCCTAGTAGTAGGAAT21680.13263913955864448No Hit
GTGTATTGATAGGATAAATACCTTACTTAGTTCAGTTTATACAGCCTTGT21640.13239441789894218No Hit
GTGCAGCCAAGGCAGGTAGAGCAAAAGAGCATGAGTGCTGAAGTCTGACA21620.13227205706909104No Hit
GGCCAAGAGAGTTTCATGCTTAACACCACAGAGATATGAAGATCACTGAG21580.13202733540938874No Hit
TTTCAGGCCAAGAGAGTTTCATGCTTAACACCACAGAGATATGAAGATCA21290.13025310337654708No Hit
CTTCTCTTCTGTAATTACCATCATGGGATATTTACTAGGAGCTTGGTGTG21200.12970247964221693No Hit
TCTCTGTGGTGTTAAGCATGAAACTCTCTTGGCCTGAAATGTGCCTCTCC21120.12921303632281234No Hit
GTGCTGAAGTCTGACAAACCAGGGCTTGAATCTCAGCTCTGCTCCTCTCC21060.12884595383325892No Hit
CACTTAAGGGGAGGCAGGCATTAGGCTGAGGGATGGGCCTAGTAGTAGGA20640.12627637640638478No Hit
GTTTATGGTCTTACAGGTGAATGCTTTGTCGTCTTCACCACCAGAACATC20600.1260316547466825No Hit
GTATTTATCCTATCAATACACAAGTGGGAGTCATTATCTCCTACTTCCCA20430.12499158769294773No Hit
GTCTTACAGGTGAATGCTTTGTCGTCTTCACCACCAGAACATCTGCTTAT20290.12413506188398969No Hit
GTGGTAGTGTGTTCTCCACGTCATTTCTGTGACAATCAAATGAGATCACA20220.12370679897951069No Hit
GCTTAACACCACAGAGATATGAAGATCACTGAGGCATCTGTGCAGCCAAG20150.12327853607503167No Hit
CTTCAGCACTCATGCTCTTTTGCTCTACCTGCCTTGGCTGCACAGATGCC20000.12236082985114805No Hit
ATATTATTCCCCACACAGGCCACAAGGTGGGAAGTAGGAGATAATGACTC19840.12138194321233886No Hit
TCACTGAGGCATCTGTGCAGCCAAGGCAGGTAGAGCAAAAGAGCATGAGT19770.12095368030785984No Hit
GTCATCCACCTCAGGTAGAAAGTTTCTGCCCTTTCCCCACTCATACCATT18680.11428501508097227No Hit
CACTGGAGATGGGGAATTATGAAATGGTATGAGTGGGGAAAGGGCAGAAA18660.11416265425112113No Hit
CTTTAAGTACGTTGCTAGTTAGTTCGAGAATACTACCAGGTGTTTATCAC18560.1135508501018654No Hit
AGCTTAGACAAGGTGCTTCACCATTCTGAGCCTCAGGCTCATCCTTGTGA18550.11348966968693981No Hit
ATTTACTAGGAGCTTGGTGTGATTATACTGTCTTTAAGTACGTTGCTAGT18080.11061419018543785No Hit
ATTCACAAGGATGAGCCTGAGGCTCAGAATGGTGAAGCACCTTGTCTAAG17970.10994120562125653No Hit
TCACCATTCTGAGCCTCAGGCTCATCCTTGTGAATTGGTGCTGGTGATAA17920.10963530354662866No Hit
GTGCTTCACCATTCTGAGCCTCAGGCTCATCCTTGTGAATTGGTGCTGGT17570.10749398902423356No Hit
GCCAGGTGGAGAGGAGCAGAGCTGAGATTCAAGCCCTGGTTTGTCAGACT17570.10749398902423356No Hit
ACACTGGAGATGGGGAATTATGAAATGGTATGAGTGGGGAAAGGGCAGAA17340.10608683948094536No Hit
GAATACTACCAGGTGTTTATCACCAGCACCAATTCACAAGGATGAGCCTG17320.10596447865109421No Hit
CCTCTCCACCTGGCTGTGTGAGCTTAGACAAGGTGCTTCACCATTCTGAG17100.10461850952273159No Hit
CAATTGTTACTTGACGGCCCTGCCTCTTTGGGCATAAAGCCCCGCACCTC17050.10431260744810371No Hit
GCCTTGTTAGCAATCTGAGCTCAACTTCCAGGCCAGCCTCCTTCCCCACC17020.10412906620332699No Hit
GAGTGGGGAAAGGGCAGAAACTTTCTACCTGAGGTGGATGACTGTCCTGG16970.10382316412869913No Hit
ATTTCAGTGTGCTAGTTTAGAAGTGGGTTCGTATAGAAAAAAACATCCTG16960.10376198371377354No Hit
CCTCTGCCCAATAAGCAGATGTTCTGGTGGTGAAGACGACAAAGCATTCA16940.10363962288392241No Hit
GCCCAATAAGCAGATGTTCTGGTGGTGAAGACGACAAAGCATTCACCTGT16930.10357844246899682No Hit
GGGATAAGATATGGGGTTCCTCTGCCCAATAAGCAGATGTTCTGGTGGTG16710.10223247334063419No Hit
GTATAAACTGAACTAAGTAAGGTATTTATCCTATCAATACACAAGTGGGA16710.10223247334063419No Hit
GGGTTCCTCTGCCCAATAAGCAGATGTTCTGGTGGTGAAGACGACAAAGC16610.10162066919137845No Hit
GTATGAGTGGGGAAAGGGCAGAAACTTTCTACCTGAGGTGGATGACTGTC16440.10058060213764371No Hit
GTACTGAACAAGTAATTTCGGTGTGCTAGTTTAGAAGTGGGTTCGTATAG16390.10027470006301582No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)