FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_H04_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_H04_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACAAAGAAAAACTCCCATTATTCAAATTAAACGTGGAAAATCATA11.7543859649122806No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT11.7543859649122806No Hit
CGTGCAAACTCCCTAACTCACAGACACCTTGTCAGACTCTGCCAGGCATT11.7543859649122806No Hit
GTGTTATTTCTTTAAACTAAGCATTTGAAATCAAAATTATTTTTTAATCT11.7543859649122806No Hit
AACATGAAATCAATCCTGCAACTCCACAAACACTCCACAACCATGAAAGA11.7543859649122806No Hit
AAGCAACAAACCACAAACAGTACTAACAAATCACAAAACAAACCTTAAAC11.7543859649122806No Hit
CAATCAACCAGTGAAGACATTGACATCATAATCTTCACAAAGCCAGATGA11.7543859649122806No Hit
TCTGAGGATTGTAAAATTATCAGGAAATCCCTCTTGGTGTTGAAGTCATG11.7543859649122806No Hit
AACAAAGACCCTGGAGAACTGGATAAGAGCCTGGCCTGAGGCGTCCCCCT11.7543859649122806No Hit
GTGTGGGCTTGAGGCCTGGCTTTTCACCCGTTCTCTCATTCATTCACCCA11.7543859649122806No Hit
AACAATGACACAGACAGACAAACAAACAAATAAAAAAAAAATCAAAACTA11.7543859649122806No Hit
AAGCACATGGACCCGTGAAGAAAATGGATTATGATGCAGTATCTGGGACT11.7543859649122806No Hit
GCTCTAACCCATTATGCAGCCCAACCAGGGGGATACTACAATGGAACAAG11.7543859649122806No Hit
CGTCCTGAGCCGGGACCTCATCCCACCCCACGTCAACTTCTCCCACCTGA11.7543859649122806No Hit
GTATTAATGTCTACTAAGACCTCGTGCAACAGTCACTCAAATGTTTGGGT11.7543859649122806No Hit
ACCTGCTTGAGAGAGCTCTGGCTGGTGCAAAGGCCCCTTAAAATCTCCGT11.7543859649122806No Hit
ACATGGTGCCTACTTCTTAGCCTGACAATGGGGTATCCAGCGGGTGAAGC11.7543859649122806No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC11.7543859649122806No Hit
GAGCACATGCTGTGTCCTAGGCATAGTCCCTGACGCACTCAAGGATCTAC11.7543859649122806No Hit
CATGAGAACCTTGCTCAAGAATACAAGACTCTGCTGCAAGAATGTCAGGC11.7543859649122806No Hit
ATTGTAACACATAACCCAGGAAAAAAGCCACACTACCACTACAAAGGGGG11.7543859649122806No Hit
TCACAACACAGGTGGTGGTTGTCAACACTGTCTAGAAGGAGTGTACAGAA11.7543859649122806No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.7543859649122806No Hit
GGCCACTAAGAGTGCAGATGGAGACATGAGACATCCGAGGGAGGGAGGAG11.7543859649122806No Hit
CTTAACTATCGGACGAATACAAATCACAAATAAACTATGAAAAACTTCAT11.7543859649122806No Hit
TATACAATCCTTGGAGCAAACCTCTAAGAAAGGTTCTGTCGCTATTCCCA11.7543859649122806No Hit
ATATAGACACAAAAGGAGCAATAGAGAGAAATCTAGCAAGGATGTCTCCC11.7543859649122806No Hit
CAACTAGTTATATTNACAAATAGTGTCACTCAGGTCTTAATTGAATCAAT11.7543859649122806No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTAGTTTCATTAACAACACAACGT11.7543859649122806No Hit
TTGCTCCAGCATGAGGACATGCTGCCGTTACAAATTTCTTCGAGTCATGA11.7543859649122806No Hit
TCTGGATGCTGAACAAAACTCCCGCTATATCCTGACCACTCTTTTATTCC11.7543859649122806No Hit
GACAATGAAGAGTTTGTCAAAGCCAAGATTTTGTCCCGGGAGGGAGCCAA11.7543859649122806No Hit
TCTGAGGAGAGAAAGTAGGAGGTGCTGGGGGGAGTTATTTAATTCCACTT11.7543859649122806No Hit
CAACCAATACATGCCTACCACACAGCCTGCAGACACTTCCCCTTCAACAC11.7543859649122806No Hit
AGTAGAAACAAGGAACTAGAAGCTCATACTGGCGAAGTAGCCCAAGAAAG11.7543859649122806No Hit
CTCCCAAATATCATTACTTTAAACAATACCAAACTAATCATGCCTTCTAA11.7543859649122806No Hit
CACAAAAACATTGTTACAAAAATATGTTCAGACACAATCCCTTCATCAAA11.7543859649122806No Hit
CCATTACACCCCTAAAAGCAAGGGCATCACAGTCAGTCCATGGGTCTCCA11.7543859649122806No Hit
GGAAAAAGCTCCTCAAAACATTCAAGTCCTTTCGATCCAGAGGCTCACTT11.7543859649122806No Hit
TTCCTTCTCAGGCTCCTTTGCGTGCCCCTCTTCATTCCTCAGGGCTCTGT11.7543859649122806No Hit
TCCCTCTAGTCCCAGCTACTCTATAGGCTGACGCAACTGACTCAGTTCAG11.7543859649122806No Hit
CAATCACCAACAAGCTCAAACCCCATCACAACTAAAAAAAGAAAAATAAC11.7543859649122806No Hit
CCTATAGGTAGATAAGGGCAAGAGATTATAAAATCTGATGGCCACGAATC11.7543859649122806No Hit
CTTTCAGGCTTGTTTGTTTTGTTTTATAGATTCAGAAATATAAAATGATA11.7543859649122806No Hit
GTCTCCTACGATGGAGTGCAGTGGTACGATCTCAGCTCACTCTAGCCCCC11.7543859649122806No Hit
ATGCAGCCCTGTCAGCGTGATACCCACCAGGGGAGGCAATTGTAGTTCCA11.7543859649122806No Hit
GGAAAAAAGAATAGAGAATTTAAATAAAAAAGTTGATGATGGTTTCCTGG11.7543859649122806No Hit
CACACGCAGTCAGCACAAGATAACCTCCCACATAAGTGAAAAATACAACA11.7543859649122806No Hit
TCTGAGGAGAGAAAGTAGGAGGTGCTGGGGGGAGTTATATAATTCCACTT11.7543859649122806No Hit
CTGAATTATCTTGCTGCCCTGCCTCTGGGAAGGTCATGTCATAACCATTT11.7543859649122806No Hit
TCTCAGCTCCCCCTGCAAGTGGCTCCACCTCTGCATCTTCTTTCCCAGAC11.7543859649122806No Hit
GAGAATGGAAGAGCGGAGTCGTAGACAGGAGCATTAGTCCCCACTGATGA11.7543859649122806No Hit
GTCATATGAATCTAAAAAAAACAGTCAATCACTCTACGAGATTAAGTCAT11.7543859649122806No Hit
GAGAAAGCCTGGGGTTTTCAGTAACTAAGATTGGCCCATGGTTCAGGCCA11.7543859649122806No Hit
GTTCATCTTCACTGTTTATTATTCCTTCATTGGAAAGCAGGACTGCGAGT11.7543859649122806No Hit
ATTATATATACCCAGCCACCTGAAACAGGTGATCTGCCAAAGTAGAAGGA11.7543859649122806No Hit
GGCATAGACCCTGCAGTCGCAGCGATCACCGTTAGACCACACGATGTCCT11.7543859649122806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)