FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_H02_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_H02_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences696474
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC21110.30309817739068506No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT18500.26562369880282677No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT17530.2516964021628948No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT16110.2313079885250562No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT12380.17775250763129707No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC11470.16468669325775262No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG11170.16037928192581488No Hit
GTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGAGCGTTCCTAGTT11100.1593742192816961No Hit
GTATGAAAAGTCCTTCAAATAGCAACTGTTCGAAATACAATTGGACCGAT10950.15722051361572723No Hit
AGCATATACTGACACATATCATTCCTATGCAAACAACATCCTAAGAACAC10920.15678977248253345No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA10340.1484621105741205No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTATTGTCAGGACC10110.14515976188630159No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG9640.13841148413293247No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG9530.13683209997788864No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA9200.1320939475127571No Hit
ATATAGACCCAAAAGGGGCAATAGAGAGAAATCTAGCAAGGATGTCTCCC9120.13094530449090705No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT8910.12793011655855066No Hit
TCCTTGTATTGATGATTCCTGTTCAACAATTGCCTCCATTTGTTGCATTG8760.1257764108925818No Hit
TATCATTCCTATGCAAACAACATCCTAAGAACACAAGAAAGTGCCTGCAA8540.12261764258249411No Hit
CTCTTGAAGAGAGGAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA8400.1206075172942565No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG8390.12046393691652524No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC8370.12017677616106273No Hit
ATACTGAGGAGTGCACATGCGGATTTGCCAGCAATAAAACCATAGAATGT8180.11744874898416884No Hit
CCTAAGATGTGGGAAATGGGAACAACCAAAGAACTGGTGCAAAACACTTA8080.11601294520685625No Hit
GTTATAATGGGAGCTAATGACGTGAGTGAATTAGAATCACAAGCACAGCT7910.11357207878542486No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA7890.11328491802996235No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA7710.11070047123079971No Hit
GGCTTTGGTCATGTCATATCCTTGTATTGATGATTCCTGTTCAACAATTG7400.10624947952113072No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA7370.10581873838793694No Hit
AACCAATAGAGTAGTAAACGAAAAAGGAGAAAGTTTCGACATGCTTTACG7370.10581873838793694No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTTGAAGAGAGGAAAG7360.1056751580102057No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC7250.10409577385516186No Hit
GCTGTAGAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCA7240.1039521934774306No Hit
CTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCTTGAGTT7240.1039521934774306No Hit
ATCATGTACCATCTCTATCCCAATACAGGGGACATCGCATTTCTTATCTT7020.10079342516734294No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)