FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_H01_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_H01_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870377
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA39310.4516433683334923No Hit
GTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAATAG26240.30147855469526424No Hit
CAACAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG23800.2734447256763448No Hit
CACGAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG21370.2455257893993063No Hit
ATGTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAAT18280.21002393215813378No Hit
CACGTGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG17430.20025804909826433No Hit
CTTCATAATCAGAACAAACATGTGATTGAGTGGAACAAGCTAGTGCTAGC15570.17888799910843234No Hit
AACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACC15480.17785396443150497No Hit
ACACAATACCTTCCCACAATAGCCACTTCATAATCAGAACAAACATGTGA14400.1654455483083767No Hit
ACACGAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATA14050.16142430234254812No Hit
AACACGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACC10700.12293523381247437No Hit
GAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCC10060.11558209833210206No Hit
GTAGTAACAAGAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAAT9890.11362892172012817No Hit
ACACGTGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATA9830.11293956526884326No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG8860.10179496930640401No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)