FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_H01_FluA_Rep1_Plate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_H01_FluA_Rep1_Plate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930159
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG16580.17824909504719086No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT16350.17577639951879195No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA16000.17201360197557622No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG13220.14212623863231985No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATC12450.13384808403724524No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC12400.13331054153107158No Hit
TCCCTGTACACATTGTGGTCATAAGTTCCATTTCTTATTGATCCTATGCA12360.13288050752613262No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC11680.12556992944217063No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG10810.11621668983474867No Hit
TTCTTAATCTGTGTCTCACTTCTTCAATTAGCCATCTTATCTCTTCAAAC10280.11051873926930772No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA10190.1095511627581951No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT10140.10901362025202144No Hit
GTACACATTGTGGTCATAAGTTCCATTTCTTATTGATCCTATGCAGGCAT10040.10793853523967406No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCCTTGTTGATTCATTG9980.10729348423226567No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGGACAA9970.10718597573103093No Hit
GAATAAGGAATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA9960.1070784672297962No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT9860.10600338221744884No Hit
ATTCAGGACCTTGAGAAATATGTTGAGGACACTAAAATAGATCTCTGGTC9810.10546583971127517No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG9730.10460577170139729No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT9730.10460577170139729No Hit
GTACTGGAGTATTCATCCACACCCATTTTGCTGACTCTTATTCCTCTCAT9510.10224058467423311No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA9510.10224058467423311No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)