FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_G09_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_G09_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1079766
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG27530.2549626493147589No Hit
TCTCTGTACACATCGTGATCATAAGTTCCATTTCTGATTGATCCTATGCA27310.2529251708240489No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT19990.1851327046786063No Hit
GTATTATTATCTCCTGAGGAGGTCAGTGAAACACAGGGAACTGAGAGACT19090.17679756539842892No Hit
GTCCCAGATATGTCAAGCAAAACACTCTGAAATTGGCAACAGGAATGAGA18570.17198170714765976No Hit
GAGTAAGGAATATCCCTAGCAGAGTAAGCATCTATTGGACAATAGTAAAA16310.1510512462885477No Hit
GTACTGGAGTATTCATCCACACCCATCTTGCTGACTCTTATTCCTCTCAT15430.14290133232570762No Hit
CTCCAGTACAGAGAGGGTGGTGGTTAGCATTGATCGGTTTTTGAGAGTTC15210.14086385383499758No Hit
GTATTGGCTTCTAATGGCCTTGGGGACTAAGGATTGAAATGGTTCAAATT14880.13780763609893254No Hit
GAATAGATTGATCGGGAAAACCAACGAGAAATTCCATCAGATTGAAAAAG14720.13632583356023434No Hit
ATTCAGGACCTTGAGAAATATGTTGAGGACACTAAAATAGATCTCTGGTC14680.13595538292555978No Hit
CACTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCATCAATGAACA13110.12141519551458371No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT13090.12122997019724643No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG12760.1181737524611814No Hit
ACCTTATATAGTCAAACCACACGAAAAGGGAATAAATTCAAATTACCTGC12710.11771068916783822No Hit
AGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAACCC12580.11650672460514593No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA11420.10576365619958399No Hit
GCTTTATTATCTGGGTGGTGTCAAATGTCCCAAGTACGTCTCTCATTTGT11230.10400401568487988No Hit
CTTTTAAGGCCATGTTTGAAGAGTCGATAGATACATTTGAAAAAAAGACG11170.10344833973286804No Hit
CTATTGGACAATAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAG11100.10280005112218758No Hit
GTCCTGAATTCTCCCTTCGACTTCTGAGAATTCTTTTTCAATCTGATGGA10980.10168869921816394No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)