FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_G05_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_G05_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1585657
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT33940.2140437686082173No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG30510.19241235651846522No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT29510.18610582238151124No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAATTGTGT27490.17336662342486425No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC25900.16333923414710746No Hit
CTCTTCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATA24620.15526687045180643No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA24070.15179827667648174No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCGGGGCC22480.14177088739872495No Hit
AAGCAGAACTAGCAGAAAAATTACACTGTTGGTTCGGTGGGAAAGAATTT21960.1384914896475089No Hit
GTGCTGACCTACTGGTTTCCTGTATGATGCACTGTTAAAACAGGCCTCAA19830.12505857193579697No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT19230.1212746514536246No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA19180.12095932474677688No Hit
ATCCAATGATGACCAATAACCCCATAGACATCTTCGGAGCTTATGTGTAC18230.11496811731667063No Hit
CCCCATAGACATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAG17740.1118779155895632No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG17720.1117517849068241No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT17610.11105806615175917No Hit
CCCATACACCGGTGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA17380.10960756330025975No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT17340.1093553019347816No Hit
CTGTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTC17030.10740027635232588No Hit
GATACAACTTAAAACAAATGCTGAAGACATAGGAACAAAAGGCCAAATGT16990.10714801498684773No Hit
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG16790.10588670815945692No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACC16640.10494072803891384No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC16600.10468846667343569No Hit
GTAGAAGTACCATACATTTGTGCAGAAGGAGAAGACCAAATTACTGTTTG16030.10109374221537193No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC16030.10109374221537193No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGG16000.1009045461912633No Hit
GTACAAACGCATCAAATGTTCAGGCTGTGAATCGTTCTGCAGCAAAAGGG15920.10040002346030699No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)