FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_G04_FluA_Rep1_Plate3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_G04_FluA_Rep1_Plate3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2158915
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC108630.5031694161187449No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG94920.4396652948356003No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA62840.29107213577190394No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT60560.2805112753396961No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT53710.24878237447977342No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT46840.2169608344932524No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT44650.20681685013073697No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT43770.20274072856041112No Hit
CCCTTAGCCTCGACATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG38820.1798125447273283No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC37840.1752732275240109No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGGCCACTT35290.1634617388827258No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA34300.15887610211610925No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTT32890.15234504369092808No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC32070.1485468395003972No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG31630.14650877871523427No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC31300.1449802331263621No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG29900.13849549426448007No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGTCAAGTGGTCAGCCTGATG29540.1368279899857104No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC28630.13261290972548712No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT27730.12844414902856297No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC27550.12761039688917813No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC27070.12538705785081858No Hit
GTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAG25210.11677161907717534No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC24520.11357556920953349No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA24460.11329765182973854No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT23900.11070375628498574No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG23660.10959208676580598No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC23660.10959208676580598No Hit
CCCTTAGTAGTATCAATGTCTCTAATCGGTTGAAGATTACACTGAAGTTC23570.10917521069611355No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG23090.10695187165775401No Hit
GTATCAATGTCTCTAATCGGTTGAAGATTACACTGAAGTTCGCTTTCAGT22990.10648867602476243No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA22760.10542332606888183No Hit
GGCAATACCCTTAGCCTCGACATCGAAACAGCCACTCTTGTTGGGAAACA22470.10408005873320626No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG22460.1040337391699071No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT22090.10231991532783828No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)