FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_G03_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_G03_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1962073
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGGCAT47520.24219282361053845No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA41740.21273418471178188No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG38310.1952526740850111No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCACTCTGCTGT32270.16446890610084336No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG27860.14199267815213806No Hit
TCTCTGTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCA25450.129709750860442No Hit
CTGTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGGC25000.12741625821261493No Hit
GAATAGATTGATCGGGAAAACCAACGAGAAATTCCATCAGATTGAAAAAG24610.12542856458449814No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG24360.12415440200237199No Hit
ATTCAGGACCTTGAGAAATATGTTGAGGACACTAAAATAGATCTCTGGTC23680.12068867977898885No Hit
GAGTAAGGAATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA22020.11222824023367121No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG21690.1105463456252647No Hit
TCTCAAAAGCACTCAAGCAGCAATCGATCAAATCAATGGAAAGCTGAATA20810.10606129333618065No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT20410.10402263320477881No Hit
CAATAGGATGGGGGCTGTAACCACCGAAGTGGCATTTGGCCTGGTATGTG19720.10050594447811065No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)