FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_G02_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_G02_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2060684
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA48120.23351469706175232No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA44890.21784028992315171No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGGACAA44070.21386102866815096No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT43770.21240520137973604No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG42610.2067760025311984No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA35650.17300080943997237No Hit
ATCACGTACTGTGTTATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC31330.15203689648679758No Hit
ATATGGGATCGAGTACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG30540.1482032179606383No Hit
TCATTACTCATATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTC28610.13883739573850234No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG27730.1345669690258186No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCGT26070.1265113913632561No Hit
GTTCAAGAGTACTCGATCCCATATTATCCATGTTCTCATTTGAAGCAATT25460.12355120921014576No Hit
CTCAATATCAATGCGGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC25360.12306593344734078No Hit
CTCATAAGCACTTCTTGTTTTCCGCCCATTCTCACCTCTCCAAAAATTTC24940.12102777524355991No Hit
TCTCTGTACACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCA24810.12039691675191344No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAGAAAAAGACG24780.12025133402307196No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCCTTGTTGATTCATTG24290.11787348278532758No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT24270.1177764276327666No Hit
GTTCCATATGTACCTGAGGTGCCACAAAACACAACAATACTGTTTGAGGT24170.11729115186996163No Hit
ATCCAGAACAGCTTGACAATAGAGAAAATGGTACTCTCTGCTTTTGATGA22830.110788456648375No Hit
GGATACTGCAGGTCCATACGCACAGGCAGGTAGGCAAGATTTGTGAGCAA22810.11069140149581401No Hit
ATCTACAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT22380.10860471571575263No Hit
GTACGTGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT22010.10680919539337423No Hit
GGTCTATTCCCACCAAGGAATATCCCTCTTTTTCGAAGTCGTACCCACTG21050.10215054807044652No Hit
CTGCAGGTCCATACGCACAGGCAGGTAGGCAAGATTTGTGAGCAACTGAT20970.10176232746020254No Hit
CCCATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTA20910.10147116200251954No Hit
GCTTATGAGAGAATGTGCAACATTCTTAAAGGAAAATTTCAAACAGCTGC20830.10108294139227557No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)