FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_G02_FluA_Rep1_Plate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_G02_FluA_Rep1_Plate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1349445
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCCTTGTTGATTCATTG23820.17651701254960372No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA22330.1654754361978443No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGGACAA20400.15117326011804855No Hit
GTATGTAATCATCGCCAGAAACATTCGAGGGTTTTGATTTTCATTCCACT18340.13590772502769657No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA17990.13331406615312222No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG17910.13272122983893378No Hit
ACCTTATATAGTCAAACCACACGAAAAGGGAATAAATTCAAATTACCTGC15390.11404688594199837No Hit
ATATAATGGGATCCCTTCTCCTTCGTGACTTGGGTCTTCAATGCTCAATT14830.1098970317426794No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAAGCTCTGTGTCTTGTGAA14730.10915598634994386No Hit
TTTCTGAACCACTCAGGTTGATTCTTTGTGATGTATGTAATCATCGCCAG14450.10708105925028438No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT14290.10589538662190753No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA14140.10478381853280423No Hit
ATATGGGATCGAGTACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG13750.10189374150113566No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)