FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_F12_FluA_Rep2_Plate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_F12_FluA_Rep2_Plate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1802416
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA63160.3504185493249061No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC43800.24300716371803177No Hit
ACCCTGGACCCTGCATAAGATGCAATAAACATGGCTACAGTCATGTTAAG41750.23163354075862622No Hit
TCCCACCTGCAGTCTGGATTCTGTCCCAATCACACACTAAAGAAGAGCCC41490.23019103248084793No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA40770.22619639417315426No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC39020.21648720384195436No Hit
ACACGATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA31880.17687370729065874No Hit
GTCACGCAGTCGGCTCTGGTTCTCCTCCCACCTCTCTGACCATTCCTTCT29390.1630589164765515No Hit
GTATATGAGGGTGTCTGAGGATTGTAAAATTATCAGGAAATCCCTCTTGG29160.16178285146159377No Hit
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA28810.15984101339535378No Hit
GAGCAAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAAGAGGC28490.15806561859193438No Hit
CTTTCTCTAGACCCTGGACCCTGCATAAGATGCAATAAACATGGCTACAG28130.15606829943808756No Hit
ACACAATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA27780.15412646137184757No Hit
CTAAAGAAGAGCCCTTGTCAAGGTTACCAGCAACCTCCACATTGGCAAAC27440.15224010439321445No Hit
GTGGCAGATGGAATCGGGGAGACTCTGCCAGCAGAACATGTGGGCAAAGC26930.14941056892526475No Hit
CTCTCTCACCCCACCTTCCTCTAGCATGCACCTGGCTGCCTTTGCCTCCC26670.1479680606474865No Hit
CTGCACTCCATCCTAGGAGACAGAGTGAGACCCTGTCTCAAAAACAAGAA26490.14696940107056308No Hit
CTCATATACAGAGCTACAATCAACCAACACTCCCATCACCACCAACTGCC26270.14574881714321222No Hit
CTCTCCACCTGCTTCAGCCCTGCCACTTACCCCAGTGAGCCTCTGCCCTT24680.13692732421372203No Hit
TCTTTAGTGTGTGATTGGGACAGAATCCAGACTGCAGGTGGGATGTGAGG23870.13243335611756665No Hit
CTTCTTTAGTGTGTGATTGGGACAGAATCCAGACTGCAGGTGGGATGTGA23760.13182306415389122No Hit
CCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGG23490.13032507478850608No Hit
GCATAAGATGCAATAAACATGGCTACAGTCATGTTAAGGGCAGAGGCTCA23190.1286606421603004No Hit
GCAGGCAGGATGTGAGGTTGCTCCCTCTCCACCTGCTTCAGCCCTGCCAC23110.12821679345944556No Hit
ATCTTGGACAGAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGC23050.12788390693380441No Hit
AAACATGGCTACAGTCATGTTAAGGGCAGAGGCTCACTGGGGTAAGTGGC22860.1268297662692741No Hit
GAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGCACATGTTTGA22680.1258311066923507No Hit
CCACATTGGCAAACCCTAAGGCCCCTTGCCAGTGCTCACCTTGCGTGGCC22530.12499889037824786No Hit
GAGTGAGACCCTGTCTCAAAAACAAGAAAGCTAAATTTGTGAACAGTTAA22230.12333445775004215No Hit
GTTAAGGGCAGAGGCTCACTGGGGTAAGTGGCAGGGCTGAAGCAGGTGGA21720.12050492228209249No Hit
GGCCAGGCGAGGTGACTCACGCCAGCAATCCCAGCACTTTGGGAGGCCGA21340.11839664095303193No Hit
GTCCAGGGTCTAGAGAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGG21290.11811923551499764No Hit
CCTTGCGTGGCCCATCTGCCGCTGCCCATGCCGCTGATGGCTTCTTCCTG20990.11645480288679194No Hit
CGATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGG20960.11628835962397138No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC20880.11584451092311653No Hit
CTATAAAAGTGAGGTGCTGGCAGGACGTACTAGCAGGCACGTGGGATGCT20730.11501229460901367No Hit
GTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCCTAGCT20390.11312593763038055No Hit
CCTTGCGTGGCCCATCTGCCGCTGCCCATGCCGCTGACGGCTTCTTCCTG20370.11301497545516684No Hit
CTAGAAAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGGCAGCCAGGT20260.11240468349149142No Hit
CTAGACAGTGTTGACAACCACCACCTGTGTTGTGATGAACTTCAACTTTT19700.10929774258550745No Hit
ATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCACACAAGT19590.10868745062183204No Hit
CCTCGTGTGGCCCATCTGCCGCTGCCCATGCCGCTGACGGCTTCTTCCTG19420.10774427213251546No Hit
GTCATGTTAAGGGCAGAGGCTCACTGGGGTAAGTGGCAGGGCTGAAGCAG19230.1066901314679852No Hit
GGTGCAGAGGGAGCAACCTCACATCCCACCTGCAGTCTGGATTCTGTCCC19180.10641272602995092No Hit
GAACCAGTTAAGAGGCTGTTTTTGATCTGGGACAGAGAGAGGGTGATGAC19170.10635724494234405No Hit
CCCTAAGGCCCCTTGCCAGTGCTCACCTTGCGTGGCCCATCTGCCGCTGC19060.10574695297866862No Hit
ATATGGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC18780.10419348252567666No Hit
CACTAAAGAAGAGCCCTTGTCAAGGTTACCAGCAACCTCCACATTGGCAA18640.10341674729918067No Hit
TTGCAATAGTGCACCAGCAGTTCACTTTCAAAGGCGGCTTCAAAATCCTC18590.10313934186114637No Hit
GGGACAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGCACATGT18470.1024735688098641No Hit
CACAAACAGCCTCTCTCACCCCACCTTCCTCTAGCATGCACCTGGCTGCC18410.10214068228422296No Hit
GAGGCAAGAGAGTCACTTGAGTATGGGAGGTGGGGGCTGCAGTGAGCTGA18230.10114202270729955No Hit
CCTTCTAGACAGTGTTGACAACCACCACCTGTGTTGTGATGAACTTCAAC18170.10080913618165839No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)