FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_F12_FluA_Rep1_Plate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_F12_FluA_Rep1_Plate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1460480
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA44460.3044204645048203No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA34950.23930488606485537No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC32580.2230773444347064No Hit
TCCCACCTGCAGTCTGGATTCTGTCCCAATCACACACTAAAGAAGAGCCC31240.2139022787028922No Hit
ACCCTGGACCCTGCATAAGATGCAATAAACATGGCTACAGTCATGTTAAG29540.20226226993865032No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC28930.19808556091148116No Hit
GTATATGAGGGTGTCTGAGGATTGTAAAATTATCAGGAAATCCCTCTTGG27800.19034837861524978No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC27430.18781496494303243No Hit
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA26810.18356978527607362No Hit
ACACGATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA25110.17192977651183172No Hit
GAGCAAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAAGAGGC22640.15501752848378617No Hit
CTAAAGAAGAGCCCTTGTCAAGGTTACCAGCAACCTCCACATTGGCAAAC21690.14851281770376862No Hit
GTCACGCAGTCGGCTCTGGTTCTCCTCCCACCTCTCTGACCATTCCTTCT20680.14159728308501315No Hit
TCTTTAGTGTGTGATTGGGACAGAATCCAGACTGCAGGTGGGATGTGAGG20560.14077563540753726No Hit
CTTTCTCTAGACCCTGGACCCTGCATAAGATGCAATAAACATGGCTACAG20000.1369412795793164No Hit
GTGGCAGATGGAATCGGGGAGACTCTGCCAGCAGAACATGTGGGCAAAGC19870.13605116126205083No Hit
CTCTCTCACCCCACCTTCCTCTAGCATGCACCTGGCTGCCTTTGCCTCCC19860.13598269062226118No Hit
ACACAATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA19840.13584574934268184No Hit
GCATAAGATGCAATAAACATGGCTACAGTCATGTTAAGGGCAGAGGCTCA18870.12920409728308502No Hit
GAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGCACATGTTTGA17970.12304173970201578No Hit
GCAGGCAGGATGTGAGGTTGCTCCCTCTCCACCTGCTTCAGCCCTGCCAC17880.12242550394390886No Hit
CTTCTTTAGTGTGTGATTGGGACAGAATCCAGACTGCAGGTGGGATGTGA17550.12016597283085013No Hit
CCACATTGGCAAACCCTAAGGCCCCTTGCCAGTGCTCACCTTGCGTGGCC17290.11838573619631904No Hit
CCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGG17070.11687938212094655No Hit
GAGTGAGACCCTGTCTCAAAAACAAGAAAGCTAAATTTGTGAACAGTTAA16940.11598926380368098No Hit
CTAGAAAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGGCAGCCAGGT16640.11393514460999123No Hit
AAACATGGCTACAGTCATGTTAAGGGCAGAGGCTCACTGGGGTAAGTGGC16580.1135243207712533No Hit
GGCCAGGCGAGGTGACTCACGCCAGCAATCCCAGCACTTTGGGAGGCCGA16350.11194949605609116No Hit
CCTTGCGTGGCCCATCTGCCGCTGCCCATGCCGCTGATGGCTTCTTCCTG16250.11126478965819457No Hit
ATCTTGGACAGAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGC16230.11112784837861525No Hit
CGATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGG16200.11092243645924628No Hit
GTTAAGGGCAGAGGCTCACTGGGGTAAGTGGCAGGGCTGAAGCAGGTGGA16140.11051161262050832No Hit
CTGCACTCCATCCTAGGAGACAGAGTGAGACCCTGTCTCAAAAACAAGAA15890.10879984662576687No Hit
GTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCCTAGCT15700.10749890446976336No Hit
CTAGACAGTGTTGACAACCACCACCTGTGTTGTGATGAACTTCAACTTTT15610.10688266871165643No Hit
CTCTCCACCTGCTTCAGCCCTGCCACTTACCCCAGTGAGCCTCTGCCCTT15490.10606102103418054No Hit
GTCCAGGGTCTAGAGAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGG15440.10571866783523226No Hit
ATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCACACAAGT15410.10551325591586329No Hit
CACAAACAGCCTCTCTCACCCCACCTTCCTCTAGCATGCACCTGGCTGCC15020.1028429009640666No Hit
GAACCAGTTAAGAGGCTGTTTTTGATCTGGGACAGAGAGAGGGTGATGAC14970.10250054776511833No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC14910.10208972392638037No Hit
CTATAAAAGTGAGGTGCTGGCAGGACGTACTAGCAGGCACGTGGGATGCT14870.10181584136722173No Hit
CCTCGTGTGGCCCATCTGCCGCTGCCCATGCCGCTGACGGCTTCTTCCTG14740.10092572304995619No Hit
GGATTGCAGTGGTACGATCTCAGCTCACTGCAGCCCCCACCTCCCATACT14720.10078878177037687No Hit
CTCAAAAGGCTGAGGCAAGAGAGTCACTTGAGTATGGGAGGTGGGGGCTG14620.10010407537248027No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)