FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_F08_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_F08_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16673
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTATCTGGAACGCACCGGGGTCCTTGACGACCTACAACGTGCGGGCTG630.37785641456246627No Hit
CCTTACAAGTTGATGCCAGTGCCCCCGGAACGGGAAACACTCAAGCCACC580.3478678102321118No Hit
CTTTACGGGAGTGCATTCTATGCTGAAACAAATTCAACATTACATTGACA540.3238769267678282No Hit
CCCTTAGGTGTGATCGTCAAGGAACCGATACCTTTAGCTGAGGTCTCTGG540.3238769267678282No Hit
TCTCAGGGAAGCGCGCAATGATCTCGTCGAGCTTCTCACGGAATACAGCA490.29388832243747376No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTA470.28189288070533197No Hit
CTATGAGATTAACTACGTGAAAGTTTAGGAGGCCCTCATGGGTAAGAAAG430.2579019972410484No Hit
CAATAACACGGAGTGCCCTATGGGGTTGAACCTTATACCTTAAAGATCAC430.2579019972410484No Hit
TCATAGCGGTGACCCCGCGATACCCATTCGACCAGAACCATTGGACGCCC390.23391111377676485No Hit
CACCTAAGGGTGATGGGTCTACCAAGGCTGTAGCCAAGGATACTGGCGCG390.23391111377676485No Hit
ATACAGCACGGTCCCCTTTATGGCACAAGTCCACATAGAGGCGCGGCTTC390.23391111377676485No Hit
CCCTTAGGTGGTTCGAGTAGCTGTGGCTCAGGCGCCTGCAACTGTTGAGG380.22791339291069393No Hit
ACCATGGACAGGCCCGCTTCAATAGCAGCGAGGTCCTCAGCGTTGCCGAT370.22191567204462304No Hit
GATGTAGAGGCACCACCACAGACCCAGATGCTCGCCCAGGCTGAAGTGAA370.22191567204462304No Hit
GTCATATGCCGCCTTAGCTCCTTCCTCAGCGAGACCCTTTCGGGCTTCTG350.20992023031248128No Hit
ATCTCATAGCGTCCAGTGTCGAGACGCTTCGTGTAGCCCATGACCTTAAA340.20392250944641035No Hit
CTTCAAGGCTGTGCAGCAGTAACCGAGATCATCGAGCTGATGCAGAACCC320.19192706771426857No Hit
GGTCTCATAAGGTGCCCGGTCGGTTTTCAGACGGTCATATGCCGCCTTAG310.18592934684819767No Hit
GTATGGTCTCCCTGGCGGCGTCTCAAGGAATGACCGAGGAAGACCTTCTG310.18592934684819767No Hit
ATGTGGACTTGTGCCATAAAGGGGACCGTGCTGTATTCCGTGAGAAGCTC310.18592934684819767No Hit
ATCTTAAAGTACGTCTGAACGCTGCCTATCTGGAACGCACCGGGGTCCTT310.18592934684819767No Hit
GTCCACACACCGAGAAGCTCATGCACGCATCTGCGCTGCCACTGAGGACC310.18592934684819767No Hit
ACATAGAGGCGCGGCTTCATAGCTTGAGAGCTCGCTTGATGGCAGGAGAC300.17993162598212678No Hit
TCCCTGAGATGACCATGCAGTCCACACACCGAGAAGCTCATGCACGCATC290.1739339051160559No Hit
CCTTAAAGCCGTTCTCTACGGCCAGCTCACGAGCCCTTCGGTGCATCATA280.16793618424998502No Hit
GTTTTGTGCACGGGTCTCATAAGGTGCCCGGTCGGTTTTCAGACGGTCAT280.16793618424998502No Hit
CCTAAGGGTGATGGGTCTACCAAGGCTGTAGCCAAGGATACTGGCGCGAC280.16793618424998502No Hit
CCTACAAGGGCAACCGTGTAGCCAAGCGCAAGCCGGTGGGTTACCCAGCG270.1619384633839141No Hit
AAAGGGGACCGTGCTGTATTCCGTGAGAAGCTCGACGAGATCATTGCGCG270.1619384633839141No Hit
ATCTGGAACGCACCGGGGTCCTTGACGACCTACAACGTGCGGGCTGGTCT260.15594074251784323No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGAAACACGTGCCCGGGTTATTA260.15594074251784323No Hit
GTAGTAACAAGAGCCGGGTTATTAGTAGAAACACGAGCCCGGGTTATTAG260.15594074251784323No Hit
CCATGTAGGACATCATGACCCGCTCAACCATGGACAGGCCCGCTTCAATA260.15594074251784323No Hit
GCTTGATACGGAGTGCTGTAGCTGGTCGAGCTGTTGTCGGACTCAGCAGG250.1499430216517723No Hit
GACCTATGTGTTTCGGGTCCAAGATGAAAGTTCCCAAACCTCAACCTCAA250.1499430216517723No Hit
ACCTAAGGGTGTCGAAGTCGGTGATGGCCCAGCAGATGGTGATGATGCAC250.1499430216517723No Hit
CCCGTAAAGTGATCTTTAAGGTATAAGGTTCAACCCCATAGGGCACTCCG240.14394530078570145No Hit
CCTTATACCTTAAAGATCACTTTACGGGAGTGCATTCTATGCTGAAACAA240.14394530078570145No Hit
CTGTAGGGCATTCTGTGTGGAGGTTTAAAACCCCTCACAATAACACGGAG240.14394530078570145No Hit
GTGTCGAGACGCTTCGTGTAGCCCATGACCTTAAAGCCGTTCTCTACGGC240.14394530078570145No Hit
GCCTTGAAGGAATCCGAGGATCTTCCCTTCAGACCAGCCCGCACGTTGTA240.14394530078570145No Hit
CCACAGACCCAGATGCTCGCCCAGGCTGAAGTGAAGACTGACGAAGCGAC240.14394530078570145No Hit
GTATGAGCATGAACTTCTGCGCGGTAAGCGTAAGGGTGAGTTCGAGACTC230.13794757991963055No Hit
GTGTTATTGTGAGGGGTTTTAAACCTCCACACAGAATGCCCTACAGTGGT230.13794757991963055No Hit
GTGCTGTAGCTGGTCGAGCTGTTGTCGGACTCAGCAGGGAACAGAGAACC230.13794757991963055No Hit
CTTTAAGATAGTCAGCAGACGCTGTTGGAATGTCAGGAACATCTTCGGGA230.13794757991963055No Hit
GGCTCAAAGTTATCAGTCGGACTACGTGCGTAGGCACATCCGGCTGGTCA220.13194985905355963No Hit
GCATTCTATGCTGAAACAAATTCAACATTACATTGACAATCCCGAAGATG220.13194985905355963No Hit
ACTCATGGGCGTCCAATGGTTCTGGTCGAATGGGTATCGCGGGGTCACCG220.13194985905355963No Hit
CTCATAAGGTGCCCGGTCGGTTTTCAGACGGTCATATGCCGCCTTAGCTC210.12595213818748877No Hit
GTTTTAAACCTCCACACAGAATGCCCTACAGTGGTTATCGCTCAGGTGTC210.12595213818748877No Hit
TTCCAACAGCGTCTGCTGACTATCTTAAAGTACGTCTGAACGCTGCCTAT210.12595213818748877No Hit
GGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTAGTAACAAGAGCCCG210.12595213818748877No Hit
ACCTACAACGTGCGGGCTGGTCTGAAGGGAAGATCCTCGGATTCCTTCAA200.11995441732141786No Hit
GCTTTAAGGTCATGGGCTACACGAAGCGTCTCGACACTGGACGCTATGAG200.11995441732141786No Hit
ATGATGCACCGAAGGGCTCGTGAGCTGGCCGTAGAGAACGGCTTTAAGGT190.11395669645534696No Hit
GCTCCAGAGGGAACAACGCAAGGTGGACTTTGGCAGCGAGGTTGTTGACG190.11395669645534696No Hit
GAATACGGGGGTGCCCTTTGAGTCAGCCTTTACGAATCGGTTTGGCCCTC190.11395669645534696No Hit
GTCCCCTTTATGGCACAAGTCCACATAGAGGCGCGGCTTCATAGCTTGAG190.11395669645534696No Hit
GTATAAGGTTCAACCCCATAGGGCACTCCGTGTTATTGTGAGGGGTTTTA190.11395669645534696No Hit
TCTCGACACTGGACGCTATGAGATTAACTACGTGAAAGTTTAGGAGGCCC180.10795897558927607No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGAAACACGAGCCCGGGTTATTA180.10795897558927607No Hit
GCCCTATGGGGTTGAACCTTATACCTTAAAGATCACTTTACGGGAGTGCA180.10795897558927607No Hit
CCTTTAGCTGAGGTCTCTGGTGCATCATCACCATCTGCTGGGCCATCACC180.10795897558927607No Hit
GTCTTCACTTCAGCCTGGGCGAGCATCTGGGTCTGTGGTGGTGCCTCTAC180.10795897558927607No Hit
CCCATGAGTGGACCCACATGGCTATCATCGTCCTCGGTGAGAACAGCGAA180.10795897558927607No Hit
GCGTAAGGGTGAGTTCGAGACTCGTACACGGGTCACCGAGAAACCTGAAG180.10795897558927607No Hit
TTCTTACCCATGAGGGCCTCCTAAACTTTCACGTAGTTAATCTCATAGCG180.10795897558927607No Hit
CTGTTGGACTCCATGTAGGACATCATGACCCGCTCAACCATGGACAGGCC180.10795897558927607No Hit
CCTTCAAGGCTGTGCAGCAGTAACCGAGATCATCGAGCTGATGCAGAACC180.10795897558927607No Hit
ATCATGACCCGCTCAACCATGGACAGGCCCGCTTCAATAGCAGCGAGGTC180.10795897558927607No Hit
GTGCTGTATTCCGTGAGAAGCTCGACGAGATCATTGCGCGCTTCCCTGAG170.10196125472320518No Hit
GTTCTCACCGAGGACGATGATAGCCATGTGGGTCCACTCATGGGCGTCCA170.10196125472320518No Hit
GCATATGACCGTCTGAAAACCGACCGGGCACCTTATGAGACCCGTGCACA170.10196125472320518No Hit
GGATTGTCAATGTAATGTTGAATTTGTTTCAGCATAGAATGCACTCCCGT170.10196125472320518No Hit
GTGTAGCCCATGACCTTAAAGCCGTTCTCTACGGCCAGCTCACGAGCCCT170.10196125472320518No Hit
ATTACATTGACAATCCCGAAGATGTTCCTGACATTCCAACAGCGTCTGCT170.10196125472320518No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)