FastQCFastQC Report
Thu 21 Apr 2022
HK3GMBGXL_n02_Flu09_F05_FluB_Rep1_Plate4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3GMBGXL_n02_Flu09_F05_FluB_Rep1_Plate4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1313668
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC24940.18985009911179992No Hit
TTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT20520.15620385059238712No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA18780.14295849484040107No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT17740.13504173048289217No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT17510.1332909075961354No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC17480.13306253939351495No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT15570.11852309716001302No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATCATTGTCAGGGCC14840.11296613756291544No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC14610.11121531467615867No Hit
CCCCCAGACCAGATGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG14380.10946449178940189No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT14200.1080942825736792No Hit
GCACTATACCTTTTCCTTTTAATTACTTTAACTGGTTTTCCTTGATAAAG13750.10466875953437245No Hit
GTACTTCCTTCATTGTTTCTTTGGCCTGAATTTCTTTTTGGTAACTGTGT13470.1025373229765816No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA13430.10223283203975435No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA13390.10192834110292706No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG13200.10048200915299757No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)